These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Hamp (Hamp1) gene. This strain may be useful for generating conditional mutations in applications related to the regulation of iron and biometal homeostasis.
Duygu Dee Harrison-Findik, University of Nebraska Medical Center
The Hamp gene encodes hepcidin, an acute phase protein important for iron homeostasis and inflammation. Mice express two Hamp genes, Hamp and Hamp2, both located on mouse chromosome 7. These mice possess loxP sites on either side of Hamp exons 2 and 3. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
When bred to a strain with widespread Cre recombinase expression, the resulting progeny mice exhibited increased selenium in the livers, increased manganese in liver and duodenum, and increased copper in the brain.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a 3’ loxP site was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into C57BL/6 embryonic stem (ES) cells (OzGene). Correctly targeted ES cells were injected into BALB/cJ blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. These mice still carry the FRT site flanked NEO selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Duygu Dee Harrison-Findik|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Hamp, hepcidin antimicrobial peptide|
|Strain of Origin||C57BL/6|
|Molecular Note||The targeting vector contains a FRT-flanked PGK-neomycin selection cassette and a 3' loxP site and is inserted downstream of exon 3, and another loxP site is inserted upstream of exon 2.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Hamp1flx/flx mouse strain in a publication, please cite the originating article(s) and include JAX stock #026872 in your Materials and Methods section.