C57BL/6-Hamp^tm1Ddhf/J

Stock number: 026872
Product name: Hamp1^flx/flx
Research areas: Metabolism Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Hamp (Hamp1) gene. This strain may be useful for generating conditional mutations in applications related to the regulation of iron and biometal homeostasis.

Detailed description

The Hamp gene encodes hepcidin, an acute phase protein important for iron homeostasis and inflammation. Mice express two Hamp genes, Hamp and Hamp2, both located on mouse chromosome 7. These mice possess loxP sites on either side of Hamp exons 2 and 3. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.

When bred to a strain with widespread Cre recombinase expression, the resulting progeny mice exhibited increased selenium in the livers, increased manganese in liver and duodenum, and increased copper in the brain.

Development

A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a 3’ loxP site was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into C57BL/6 embryonic stem (ES) cells (OzGene). Correctly targeted ES cells were injected into BALB/cJ blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. These mice still carry the FRT site flanked NEO selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Hamp^tm1Ddhf
Name: targeted mutation 1, Duygu Dee Harrison-Findik
MGI accession ID: MGI:5634088
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Hamp
Marker name: hepcidin antimicrobial peptide
Chromosome: 7
Strain of origin: C57BL/6
Molecular note: The targeting vector contains a FRT-flanked PGK-neomycin selection cassette and a 3' loxP site and is inserted downstream of exon 3, and another loxP site is inserted upstream of exon 2.