D2.FVB-Tg(GFAPGFP)14Mes/SjJ

Stock number: 026856
Product name: hGFAP-GFP
Research areas: Neurobiology Research, Research Tools

Description

Transgenic mice overexpress Green Fluorescent Protein under the control of the astrocyte-specific glial fibrillary acidic protein promoter. These mice provide a method to follow changes in astrocyte morphology during development or disease processes.

Detailed description

Transgenic mice overexpress Green Fluorescent Protein under the control of the astrocyte-specific glial fibrillary acidic protein promoter. Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition retinal Mullers cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors. These mice provide a method to follow changes in astrocyte morphology during development or disease processes.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

Transgenic mice express a mutant form of GFP (hGFP-S65T) under the control of the human astrocyte-specific glial fibrillary acidic protein (GFAP) promoter.

In this line, animals from Stock No. 003257 were backcrossed to DBA/2J for at least 10 generations by the donating laboratory.

Allele

Symbol: Tg(GFAPGFP)14Mes
Name: transgene insertion 14, Albee Messing
MGI accession ID: MGI:3056685
Generation method: Transgenic
Attributes: Reporter
Synonyms: GFAP::GFP; GFAP-GFP; GFAP^P-GFP; hGFAP::eGFP; hGFAP-GFP
Marker symbol: Tg(GFAPGFP)14Mes
Marker name: transgene insertion 14, Albee Messing
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition, retinal Muller cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors.
Molecular note: The transgene contains a mutant form of Green Fluorescent Protein (hGFP-S65T) under the control of the human astrocyte-specific glial fibrillary acidic protein (GFAP) promoter.
General note: Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition retinal Mullers cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors.