Transgenic mice overexpress Green Fluorescent Protein under the control of the astrocyte-specific glial fibrillary acidic protein promoter. These mice provide a method to follow changes in astrocyte morphology during development or disease processes.
Dr. Simon John, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
This strain may be carry the retinal degeneration allele Pde6brd1.
Transgenic mice overexpress Green Fluorescent Protein under the control of the astrocyte-specific glial fibrillary acidic protein promoter. Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition retinal Mullers cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors. These mice provide a method to follow changes in astrocyte morphology during development or disease processes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Transgenic mice express a mutant form of GFP (hGFP-S65T) under the control of the human astrocyte-specific glial fibrillary acidic protein (GFAP) promoter.
In this line, animals from Stock No. 003257 were backcrossed to DBA/2J for at least 10 generations by the donating laboratory.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition, retinal Muller cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors. |
Allele Name | transgene insertion 14, Albee Messing |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | GFAP::GFP; GFAPP-GFP; GFAP-GFP; hGFAP::eGFP; hGFAP-GFP |
Gene Symbol and Name | Tg(GFAPGFP)14Mes, transgene insertion 14, Albee Messing |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition, retinal Muller cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors. |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition retinal Mullers cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors. |
Molecular Note | The transgene contains a mutant form of Green Fluorescent Protein (hGFP-S65T) under the control of the human astrocyte-specific glial fibrillary acidic protein (GFAP) promoter. |
Mutations Made By | Dr. Albee Messing, University of Wisconsin-Madison |
Hemizygotes are viable and fertile.
When using the hGFAP-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #026856 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(GFAPGFP)14Mes |
Frozen Mouse Embryo | D2.FVB-Tg(GFAPGFP)14Mes/SjJ | $2595.00 |
Frozen Mouse Embryo | D2.FVB-Tg(GFAPGFP)14Mes/SjJ | $2595.00 |
Frozen Mouse Embryo | D2.FVB-Tg(GFAPGFP)14Mes/SjJ | $3373.50 |
Frozen Mouse Embryo | D2.FVB-Tg(GFAPGFP)14Mes/SjJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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