Exon 2 of the mouse Zbtb46 gene is flanked by loxP sites to create this conditional zDClox allele. Cre-mediated excision of the floxed region creates a null allele, useful in studies of classical dendritic cells (cDCs).
Michel C Nussenzweig, The Rockefeller University
Zbtb46 (zinc finger and BTB domain containing 46; also known as zDC or Btbd4) is a classical dendritic cell (cDC)-restricted zinc finger transcription factor that is not expressed by other immune cell populations, including plasmacytoid DCs, monocytes, or macrophages. It acts primarily as a negative regulator of cDC gene expression.
Exon 2 of the mouse Zbtb46 gene, encoding the translational start site and the BTB protein-binding domain, is flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region creates a knockout allele.
When crossed with EIIa-Cre mice, null mutant animals can be produced. zDC deficiency alters the cDC subset composition in the spleen in favor of CD8+ DCs, up-regulates activation pathways in steady-state cDCs, including elevated MHC II expression, and enhances cDC production of vascular endothelial growth factor leading to increased vascularization of skin-draining lymph nodes.
Albino C57BL/6J-Tyrc-2J-derived CY2.4 embryonic stem cells were used to generate targeted mutation mice in which exon 2, encoding the translational start site and the BTB protein-binding domain, is flanked by loxP sites. This strain was backcrossed to black C57BL/6 mice for approximately 4 generations by the donating laboratory.
|Allele Name||targeted mutation 2, Michel C Nussenzweig|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Zbtb46, zinc finger and BTB domain containing 46|
|Strain of Origin||B6(Cg)-Tyrc-2J|
|Molecular Note||Exon 2 of the mouse Zbtb46 gene, encoding the translational start site and the BTB protein-binding domain, is flanked by loxP sites.|
Heterozygous and homozygous floxed mice are viable and fertile.
When using the B6(Cg)-Zbtb46tm2Mnz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026850 in your Materials and Methods section.