GM-CSF KO mice have a neomycin cassette replacing exons 3 and 4 of the colony stimulating factor 2 (granulocyte-macrophage) (Csf2) gene. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Homozygous mice are viable and fertile. Aged GM-CSF KO mice show impaired phagocytosis of apoptotic cells and develop a systemic lupus erythematosis (SLE)-like disorder. They also exhibit immune-mediated glomerulonephritis with B and T cell aggregates in the renal pelvis and liver. These mice show an increase in intensity of lung pathology resembling pulmonary alveolar proteinosis after infection with Pneumocystis carinii pneumonia.
A targeting vector was designed to replace exons 3 and 4 of the colony stimulating factor 2 (granulocyte-macrophage) (Csf2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred together. GM-CSF mice were bred to C57BL/6 mice for at least 9 generations by the donating lab (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Richard C Mulligan|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Csf2-; Csf2tm1Jaw; GM-; GM-CSF-; GM-CSF KO|
|Gene Symbol and Name||Csf2, colony stimulating factor 2 (granulocyte-macrophage)|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Exons 3 and 4 and flanking intronic sequence were replaced by a neomycin cassette.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S-Csf2tm1Mlg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026812 in your Materials and Methods section.