B6.129S-Csf2^tm1Mlg/J

Stock number: 026812
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

GM-CSF KO mice may be useful for studying the development and activation of hematopoietic cells.

Detailed description

GM-CSF KO mice have a neomycin cassette replacing exons 3 and 4 of the colony stimulating factor 2 (granulocyte-macrophage) (Csf2) gene. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Homozygous mice are viable and fertile. Aged GM-CSF KO mice show impaired phagocytosis of apoptotic cells and develop a systemic lupus erythematosis (SLE)-like disorder. They also exhibit immune-mediated glomerulonephritis with B and T cell aggregates in the renal pelvis and liver. These mice show an increase in intensity of lung pathology resembling pulmonary alveolar proteinosis after infection with Pneumocystis carinii pneumonia.

Development

A targeting vector was designed to replace exons 3 and 4 of the colony stimulating factor 2 (granulocyte-macrophage) (Csf2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred together. GM-CSF mice were bred to C57BL/6 mice for at least 9 generations by the donating lab (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Csf2^tm1Mlg
Name: targeted mutation 1, Richard C Mulligan
MGI accession ID: MGI:1930996
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Csf2
Marker name: colony stimulating factor 2 (granulocyte-macrophage)
Chromosome: 11
Strain of origin: 129S2/SvPas
Molecular note: Exons 3 and 4 and flanking intronic sequence were replaced by a neomycin cassette.