Zfp318Fl/Fl floxed mice possess loxP sites flanking exons 1-2. They may be useful for studying B lymphocyte-specific antibody production.
John H. Weis, University of Utah
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Zfp318 | zinc finger protein 318 |
Zfp318Fl/Fl floxed mice possess loxP sites flanking exons 1-2, including the promoter, of the zinc finger protein 318 (Zfp318) gene. ZFP318 is a transcriptional regulatory protein expressed during spermatogenesis in the testes where it is augments ligand-dependent androgen receptor (AR) control. ZFP318 is also expressed in B lymphocytes during the transition of naive B cells from an immature to mature state. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 1-2 deleted in the cre-expressing tissues.
When bred to B6.Cg-Tg(Vav1-cre)A2Kio/J mice (Stock No. 008610) expressing Cre recombinase in hematopoietic cells and their progenitors, maturing and mature splenic naive B cells in the offspring mice have an elevated level of IgM gene products and a loss of IgD products. Aside from a slight reduction in the transitional 2 (T2) subset, B cells developed from marrow precursors are identical to those of wild type mice.
A targeting vector was designed to insert a loxP site upstream of the promoter, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2 of the zinc finger protein 318 (Zfp318) gene. The construct was electroporated into 129/Sv embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Flp transgenic mice on a C57BL/6 background to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene, resulting in a colony of Zfp318Fl/Fl mice. Mice were bred to C57BL/6J mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, John Weis |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Zfp318Fl |
Gene Symbol and Name | Zfp318, zinc finger protein 318 |
Gene Synonym(s) | |
Strain of Origin | 129/Sv |
Chromosome | 17 |
Molecular Note | The targeting vector was designed to insert a loxP site upstream of the promoter, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2 of the gene. FLP mediated recombination removed the FRT-flanked neo cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129(Cg)-Zfp318tm1.1Jhws/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026747 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Zfp318<tm1.1Jhws> |
Frozen Mouse Embryo | B6.129(Cg)-Zfp318<tm1.1Jhws>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Zfp318<tm1.1Jhws>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Zfp318<tm1.1Jhws>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Zfp318<tm1.1Jhws>/J Frozen Embryo | $3373.50 |
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