B6.129(Cg)-Zfp318^tm1.1Jhws/J

Stock number: 026747
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Zfp318^Fl/Fl floxed mice possess loxP sites flanking exons 1-2. They may be useful for studying B lymphocyte-specific antibody production.

Detailed description

Zfp318^Fl/Fl floxed mice possess loxP sites flanking exons 1-2, including the promoter, of the zinc finger protein 318 (Zfp318) gene. ZFP318 is a transcriptional regulatory protein expressed during spermatogenesis in the testes where it is augments ligand-dependent androgen receptor (AR) control. ZFP318 is also expressed in B lymphocytes during the transition of naive B cells from an immature to mature state. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 1-2 deleted in the cre-expressing tissues.

When bred to B6.Cg-Tg(Vav1-cre)A2Kio/J mice (Stock No. 008610) expressing Cre recombinase in hematopoietic cells and their progenitors, maturing and mature splenic naive B cells in the offspring mice have an elevated level of IgM gene products and a loss of IgD products. Aside from a slight reduction in the transitional 2 (T2) subset, B cells developed from marrow precursors are identical to those of wild type mice.

Development

A targeting vector was designed to insert a loxP site upstream of the promoter, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2 of the zinc finger protein 318 (Zfp318) gene. The construct was electroporated into 129/Sv embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Flp transgenic mice on a C57BL/6 background to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene, resulting in a colony of Zfp318^Fl/Fl mice. Mice were bred to C57BL/6J mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Zfp318^tm1.1Jhws
Name: targeted mutation 1.1, John Weis
MGI accession ID: MGI:5606279
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Zfp318
Marker name: zinc finger protein 318
Chromosome: 17
Strain of origin: 129/Sv
Molecular note: The targeting vector was designed to insert a loxP site upstream of the promoter, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2 of the gene. FLP mediated recombination removed the FRT-flanked neo cassette.