These mutant floxed mice possess loxP sites flanking exon 2 of the Mir155 gene. This strain may be useful for generating conditional mutations in applications related to the regulation of T follicular helper cells and germinal center B cells development, inflammation and immunity.
Gwenn Garden, University of Washington
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Mir155 | microRNA 155 |
Mir155 encodes an evolutionarily conserved multifunctional miRNA that is involved in regulation of hematopoiesis, inflammation and immunity.
These mice possess loxP sites on either side of the exon 2 of Mir155. Exon 2 includes sequence that encodes the hairpin sequence. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the coding exon deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in T cells, this mutant mouse strain may be useful in studies of chronic, low-grade inflammation and development of T follicular helper (Tfh) cells, and germinal center (GC) B cells.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of the coding exon. This construct was electroporated into unspecified C57BL/6 derived embryonic stem (ES) cells which were transiently transfected with a FLPe recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were backcrossed to C57BL/6 for 7 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1.1, Gwenn Garden |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | MiR155fl |
Gene Symbol and Name | Mir155, microRNA 155 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 16 |
Molecular Note | The targeting vector contains an FRT-flanked PGK-neomycin selection cassette, followed by a loxP site inserted downstream of exon 2 of the targeted gene, and another loxP site inserted upstream of this coding exon. The neomycin cassette was deleted by transient transfection of a flp recombinase expression vector in the ES cells. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Mir155fl/fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #026700 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Mir155<tm1.1Ggard> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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