RGS4fl floxed mice possess loxP sites flanking exons 2-5 of the regulator of G-protein signaling 4 (Rgs4) gene. RGS4 is a GTPase regulatory protein that negatively regulates vascular tone, protects vascular smooth muscle cells from to atherosclerosis, contributes to a decrease in angiotensin-dependent superoxide production, and prevents vascular oxidation. It is also widely expressed in the central nervous system and is a candidate regulator of several types of neurotransmission acting through G protein-coupled receptors. RGS4 has been shown to modulate neurotransmitter receptors and opioid-induced adaptations. RGS4 has been associated with schizophrenia, Parkinson’s disease, and opioid drug tolerance. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-5 deleted in cre-expressing tissues.
For example, when crossed to B6.FVB-Tg(Myh11-cre/ERT2)1Soff/J mice (Stock No. 019079), expressing Cre recombinase in smooth muscle cells, acute renal ischemic injury is prolonged.
A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 5 of the regulator of G-protein signaling 4 (Rgs4) gene. The construct was electroporated into embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with C57BL/6 mice for at least 20 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Andrew Siedlecki|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Rgs4, regulator of G-protein signaling 4|
|Strain of Origin||Not Specified|
|Molecular Note||A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 5 of the gene.|
When maintaining a live colony, homozygotes may be bred together.
When using the B6.Cg-Rgs4tm1Sdlk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026695 in your Materials and Methods section.