These Lphn3flox mice may be useful for generating tissue specific deletions of Adgrl3 in neuronal cells.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Lphn3flox mice possess loxP sites flanking the coding exon of the adhesion G protein-coupled receptor L3 (Adgrl3) gene. Adgrl3 encodes LPHN3, a neuronal adhesion G protein-coupled receptor involved in cell adhesion and signal transduction in the brain and other tissues. This gene has also been identified as a candidate gene for attention deficit hyperactivity disorder (ADHD). Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have the coding region deleted in the cre-expressing tissues
A targeting vector was designed to insert a double hemagglutine (HA) tag into the 3’ end of the coding exon of the adhesion G protein-coupled receptor L3 (Adgrl3) (formerly known as latrophilin 3 (Lphn3))gene. A loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, was inserted upstream of the coding exon, and a second loxP site was inserted downstream. The construct was electroporated into (129S6 x C57BL/6J) F1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred together. Lphn3flox mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Adgrl3, adhesion G protein-coupled receptor L3|
|Strain of Origin||(129S6/SvEvTac x C57BL/6)F1|
|Molecular Note||A targeting vector was designed to insert a double hemagglutinin (HA) tag into the 3'end of the coding exon of the gene. A loxP site, followed by a frt-flanked neomycin resistance cassette, was inserted upstream of the coding exon, and a second loxP site was inserted downstream.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Lphn3flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #026684 in your Materials and Methods section.