Exon 2 of the mouse Dsp (desmoplakin) gene is floxed by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region produces a knockout allele, useful in studies of cellular adhesion.
Elaine Fuchs, The Rockefeller University
The Dsp (desmoplakin) gene encodes a cytoskeletal linker protein that plays an integral role in epidermal integrity.
Exon 2 of the mouse Dsp gene is flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region results in a knockout allele, as determined by western blot. Homozygous floxed mice are viable, fertile, and overtly normal.
Newborn animals homozygous for an epidermis-specific knockout of the gene (made through crosses with keratin 14-cre mice, e.g. Stock No. 005107) show dramatic skin defects. The fragile skin has roughly normal numbers of desmosomes, but they lack keratin filament attachments, which compromise their function. Adherens junctions are also reduced.
|Allele Name||targeted mutation 1, Elaine Fuchs|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Dsp, desmoplakin|
|Strain of Origin||129|
|General Note||ES cell line = GS1 (129/Sv) or RW-4 (129X1/SvJ).|
|Molecular Note||A floxed neomycin and thymidine kinase expression cassette were inserted 3' to exon 2, and a single loxP site was inserted 5' of exon 2. Cre-mediated recombination in ES cells removed the neo and tk sequences, leaving a single loxP site 3' to exon 2.|
Homozygous and heterozygous floxed mice are viable and fertile.
When using the B6.129-Dsptm1Efu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026665 in your Materials and Methods section.