STOCK Tg(Pbsn-cre)4Prb/J

Stock number: 026662
Product name: PB-Cre4
Research areas: Cancer Research, Internal/Organ Research, Research Tools

Description

Mice harboring the PB-Cre4 transgene have the rat Pbsn, probasin, gene promoter directing expression of Cre recombinase to epithelial cells of the prostate.

Detailed description

These transgenic mice express Cre recombinase under the control of the rat Pbsn, probasin, gene promoter, in the prostate epithelium. Cre recombinase mRNA is detected in prostate tissue by RT-PCR, while none is detected in testes, seminal vesicles, liver or kidney. Cre recombinase activity is detected in more than 95% of the lateral lobe epithelium, approximately 50% of the ventral lobe epithelium, approximately 10% of the dorsal lobe epithelim and 5% of the anterior lobe epithelium. Recombinase activity is detected in less than 5% of the seminal vesicle stroma, testicular tubules, and within oocytes in the ovary. No recombinase activity is detected in liver, kidney, lung, heart, spleen, brain, salivary gland, skin, bladder, uterus or mammary gland. Mice hemizygous for the transgenic insert are viable and fertile. It is not known if homozygotes are viable. When breeding with floxed mice, to increase the likelihood of prostate specific cre recombination, the Tg(Pbsn-cre)4Prb transgene must be inherited from the male only. Residual Cre recombinase activity in oocytes of female carriers may cause unintended germline Cre-mediated recombination.

When bred to a strain with a floxed Rb1 strain (see Stock No. 026563), and an Akap12 knock out strain (see Stock No. 026228), this mutant mouse strain may be useful in studies of prostate cancer (see Stock No. 026229 for the triple mutant line).

Development

A transgenic construct containing sequence encoding Cre recombinase under the control of the rat Pbsn, probasin, gene promoter with two tandem repeats of the androgen responsive region, was injected into fertilized (C57BL/6 x DBA/2)F1 mouse eggs. Founder line 1 was subsequently established and maintained on a mixed B6;D2 background. The transgenic mice were then crossed to other mutant lines on various genetic backgrounds, including 129 and FVB. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Pbsn-cre)4Prb
Name: transgene insertion 4, Pradip Roy-Burman
MGI accession ID: MGI:2385927
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Pbsn-cre)4Prb
Marker name: transgene insertion 4, Pradip Roy-Burman
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is expressed in the prostate epithelium.
Molecular note: The transgene is composed of the cre recombinase gene under the control of a composite promoter derived from rat Pbsn. The rat Pbsn promoter drives postnatal transgene expression in the prostatic epithelium. The transgene is expressed postnatally in prostatic epithelium with the highest level of expression in the lateral lobe of the prostate gland. A very low level of transgene expression was detected in the seminal vesicles, testes, and ovaries. No transgene expression was detected in any of the other tissue types examined.