Mice harboring the PB-Cre4 transgene have the rat Pbsn, probasin, gene promoter directing expression of Cre recombinase to epithelial cells of the prostate.
Irwin H. Gelman, Roswell Park Cancer Institute
Genetic Background | Generation |
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?+N2F7
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
These transgenic mice express Cre recombinase under the control of the rat Pbsn, probasin, gene promoter, in the prostate epithelium. Cre recombinase mRNA is detected in prostate tissue by RT-PCR, while none is detected in testes, seminal vesicles, liver or kidney. Cre recombinase activity is detected in more than 95% of the lateral lobe epithelium, approximately 50% of the ventral lobe epithelium, approximately 10% of the dorsal lobe epithelim and 5% of the anterior lobe epithelium. Recombinase activity is detected in less than 5% of the seminal vesicle stroma, testicular tubules, and within oocytes in the ovary. No recombinase activity is detected in liver, kidney, lung, heart, spleen, brain, salivary gland, skin, bladder, uterus or mammary gland. Mice hemizygous for the transgenic insert are viable and fertile. It is not known if homozygotes are viable. When breeding with floxed mice, to increase the likelihood of prostate specific cre recombination, the Tg(Pbsn-cre)4Prb transgene must be inherited from the male only. Residual Cre recombinase activity in oocytes of female carriers may cause unintended germline Cre-mediated recombination.
When bred to a strain with a floxed Rb1 strain (see Stock No. 026563), and
an Akap12 knock out strain (see Stock No. 026228), this mutant mouse strain may be useful in studies of prostate cancer (see Stock No. 026229 for the triple mutant line).
A transgenic construct containing sequence encoding Cre recombinase under the control of the rat Pbsn, probasin, gene promoter with two tandem repeats of the androgen responsive region, was injected into fertilized (C57BL/6 x DBA/2)F1 mouse eggs. Founder line 1 was subsequently established and maintained on a mixed B6;D2 background. The transgenic mice were then crossed to other mutant lines on various genetic backgrounds, including 129 and FVB. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is expressed in the prostate epithelium. |
Allele Name | transgene insertion 4, Pradip Roy-Burman |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | ARR2PB-Cre; PB-Cre; PbCre4; PB-Cre4 |
Gene Symbol and Name | Tg(Pbsn-cre)4Prb, transgene insertion 4, Pradip Roy-Burman |
Gene Synonym(s) | |
Promoter | Pbsn, probasin, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is expressed in the prostate epithelium. |
Strain of Origin | (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | The transgene is composed of the cre recombinase gene under the control of a composite promoter derived from rat Pbsn. The rat Pbsn promoter drives postnatal transgene expression in the prostatic epithelium. The transgene is expressed postnatally in prostatic epithelium with the highest level of expression in the lateral lobe of the prostate gland. A very low level of transgene expression was detected in the seminal vesicles, testes, and ovaries. No transgene expression was detected in any of the other tissue types examined. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to inbred mice. The Donating Investigator has not attempted to make the strain homozygous. When breeding with floxed mice, to increase the likelihood of prostate specific cre recombination, the Tg(Pbsn-cre)4Prb transgene must be inherited from the male only.
When using the PB-Cre4 mouse strain in a publication, please cite the originating article(s) and include JAX stock #026662 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous or wildtype for Tg(Pbsn-cre)4Prb |
Frozen Mouse Embryo | STOCK Tg(Pbsn-cre)4Prb/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pbsn-cre)4Prb/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pbsn-cre)4Prb/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Pbsn-cre)4Prb/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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