Exons 4-6 and the coding region of exon 7 were replaced by a loxP-flanked neomycin cassette in this targeted allele of Cyp26b1. Homozygotes are born but die shortly after birth and demonstrate a number of developmental defects. Embryonic testicular germ cells fail to arrest in G0 and some exhibit meiotic, and apoptotic features.
David Page, Whitehead Institute for Biomedical Research
Cyp26b1 (cytochrome P450, family 26, subfamily b, polypeptide 1; also called cytochrome P450 26B1) encodes an enzyme that is involved in the inactivation of all-trans-retinoic-acid, a key component required for germ cell meiosis in mice. CYP26B1 is normally expressed in fetal testes to delay meiosis in males, negatively regulating pathways that involve retinoic acid, Stra8 and Rec8.
Exons 4-6 and the coding region of exon 7 were replaced by a loxP-flanked neomycin cassette in this targeted allele of Cyp26b1. Protein expression has not been assayed. Homozygous mice are born, but die shortly after birth. The embryos exhibit developmental defects involving several tissues, including the lungs, limbs, chin, and germ cells. Embryonic testicular germ cells fail to arrest in G0 and some exhibit meiotic, and apoptotic features. Dividing germ cells can be found in embryonic testes as late as E18.5. Quantitative real-time PCR shows an increase in testicular expression levels of STRA8.
Exons 4-6 and the coding region of exon 7 were replaced by a loxP-flanked PGK-neomycin cassette via homologous recombination in (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells. Resultant animals were bred to outbred CD1 animals for 5 generations by the donating laboratory.
|Allele Name||targeted mutation 1, David C Page|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, David C Page; Cyp26b1tm1Dcp|
|Gene Symbol and Name||Cyp26b1, cytochrome P450, family 26, subfamily b, polypeptide 1|
|Gene Synonym(s)||CP26; retinoic acid B1; P450RAI-2; P450RAI2; RHFCA; CYP26A2|
|Strain of Origin||(C57BL/6 x 129S4/SvJae)F1|
|Molecular Note||Exons 4-6 and the coding region of exon 7 were replaced by a loxP-flanked PGK-neomycin cassette via homologous recombination.|
Heterozygotes are viable and fertile. Homozygotes are born, but die shortly after birth.
When using the STOCK Cyp26b1tm1Dcp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026629 in your Materials and Methods section.
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