B6;FVB-Tg(KRT5-Venus)1Sgkd/J

Stock number: 026529
Product name: K5-Venus

Description

This strain is genotyped using generic Venus primers. There is no allele specific assay available at this time.

These K5-Venus transgenic mice express the yellow fluorescent protein Venus variant, under the control of the bovine keratin 5 promoter, and have applications in lineage tracing of the epithelial progenitor cell population in the developing submandibular gland and sublingual gland.

Detailed description

Keratin 5, also known as Cytokeratin-5 protein, is a type II cytokeratin specifically expressed in the basal layer of the epidermis, and is a basal epithelial cell marker in adult mouse salivary glands. These transgenic mice express the Venus variant of yellow fluorescent protein under the control of the bovine, keratin 5, promoter. Fluorescence is detected in the epithelial progenitor cells in the end buds and ducts of the developing submandibular gland and sublingual gland. The Donating Investigator reports transgene expression was detected in all epithelial tissues including: skin, tongue, oral cavity lung, and forestomach.

Transgene expression mimics the endogenous mouse Krt5 gene expression pattern. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background. Mice that are homozygous for the transgene are viable and fertile on the FVB background. The Donating Investigator reports that homozygotes exhibit a stronger Venus YFP expression compared to hemizgyotes.

Development

A transgenic construct containing the Venus variant of yellow fluorescent protein under the control of the 5.4 kb bovine, keratin 5 promoter (including 5’ regulatory and promoter regions from -5,300 bp to +138 bp), and a polyadenylation signal, was injected into fertilized FVB/N blastocysts. Founder line 1 was subsequently established. The mice were then backcrossed to C57BL/6 for 3 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(KRT5-Venus)1Sgkd
Name: transgene insertion 1, Silvio Gutkind
MGI accession ID: MGI:5755396
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(KRT5-Venus)1Sgkd
Marker name: transgene insertion 1, Silvio Gutkind
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: The Venus variant of yellow fluorescent protein is expressed in the epithelial progenitor cells in the end buds and ducts of the developing submandibular gland and sublingual gland.
Molecular note: The transgenic construct contains the Venus variant of yellow fluorescent protein under the control of the 5.4 kb bovine, keratin 5 promoter (including 5' regulatory and promoter regions from -5,300 bp to +138 bp), and a polyadenylation signal.