TPOCreER transgenic mice express Cre-ERT2 under the direction of the human thyroid peroxidase promoter. They are useful for applications requiring tamoxifen-induced deletion of floxed sequences in the thyroid.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Expression of creERT2 in TPOCreER transgenic mice is directed by the human thyroid peroxidase (TPO) promoter in the thyroid. TPO encodes an enzyme essential for thyroid gland function. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. Homozygotes are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.
For example, when bred to
B6.129P2(Cg)-Braftm1Mmcm/J mice (Stock No. 017837), which express the V600E mutant Braf allele after cre recombination, resulting TB offspring develop papillary thyroid carcinomas (PTCs) in a tamoxifen-dependent manner. These mice, however, lack extrathyroidal invasion and cervical lymph node metastases.
When TB mice are further crossed to B6.129S4-Trp53tm3.1Tyj/J mice (Stock No. 008182), which express the R270H missense mutant Trp53 allele, resulting offspring displayed accelerated and aggressive PTCs and had decreased survival time after tamoxifen induction.
A transgenic vector was generated including the human thyroid peroxidase (TPO) promoter driving expression of a creERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain). This transgene was microinjected into fertilized C57BL/6NTac oocytes. Offspring from founder line 1139 have been maintained on a C57BL/6J background after crosses to numerous floxed alleles on congenic C57BL/6J backgrounds. The donating investigator reports that the contaminating alleles have been removed from this TPOCreER transgenic line. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | Cre-ERT2 fusion protein is expressed under direction of the human TPO promoter in the thyroid. |
Allele Name | transgene insertion 1139, Tyler Jacks |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | TPOCreER |
Gene Symbol and Name | Tg(TPO-cre/ERT2)1139Tyj, transgene insertion 1139, Tyler Jacks |
Gene Synonym(s) | |
Promoter | TPO, thyroid peroxidase, human |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Cre-ERT2 fusion protein is expressed under direction of the human TPO promoter in the thyroid. |
Strain of Origin | C57BL/6NTac |
Chromosome | UN |
Molecular Note | A construct with the human thyroid peroxidase promoter (TPO) and an inducible cre/estrogen receptor ligand binding domain fusion (cre/ERT2) was used to generate transgenic founders. Two founders were obtained and one (#1139) was used for further analyses. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026512 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(TPO-cre/ERT2)1139Tyj |
Frozen Mouse Embryo | B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J Frozen Embryo | $3373.50 |
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