Overview

Also Known As:TPO-CreER

This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers can register interest.

TPOCreER transgenic mice express Cre-ER^T2 under the direction of the human thyroid peroxidase promoter. They are useful for applications requiring tamoxifen-induced deletion of floxed sequences in the thyroid.

Donating Investigator

Dr. Tyler Jacks, Massachusetts Institute of Technology

Genetic overview

Genetic Background	Generation

Tg(TPO-cre/ERT2)1139Tyj

Allele Type
Transgenic (Recombinase-expressing, Inducible)

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Research Applications

Research Tools
Internal/Organ Research

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Details

Detailed Description

Expression of creER^T2 in TPOCreER transgenic mice is directed by the human thyroid peroxidase (TPO) promoter in the thyroid. TPO encodes an enzyme essential for thyroid gland function. Restricted to the cytoplasm, Cre-ER^T2 can only gain access to the nuclear compartment after exposure to tamoxifen. Homozygotes are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.

For example, when bred to
B6.129P2(Cg)-Braf^tm1Mmcm/J mice (Stock No. 017837), which express the V600E mutant Braf allele after cre recombination, resulting TB offspring develop papillary thyroid carcinomas (PTCs) in a tamoxifen-dependent manner. These mice, however, lack extrathyroidal invasion and cervical lymph node metastases.

When TB mice are further crossed to B6.129S4-Trp53^tm3.1Tyj/J mice (Stock No. 008182), which express the R270H missense mutant Trp53 allele, resulting offspring displayed accelerated and aggressive PTCs and had decreased survival time after tamoxifen induction.

Development

A transgenic vector was generated including the human thyroid peroxidase (TPO) promoter driving expression of a creER^T2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain). This transgene was microinjected into fertilized C57BL/6NTac oocytes. Offspring from founder line 1139 have been maintained on a C57BL/6J background after crosses to numerous floxed alleles on congenic C57BL/6J backgrounds. The donating investigator reports that the contaminating alleles have been removed from this TPOCreER transgenic line. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.

Expression Data

Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre-ERT2 fusion protein is expressed under direction of the human TPO promoter in the thyroid.

Control Suggestions

Noncarrier

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

McFadden DG; Vernon A; Santiago PM; Martinez-McFaline R; Bhutkar A; Crowley DM; McMahon M; Sadow PM; Jacks T. 2014. p53 constrains progression to anaplastic thyroid carcinoma in a Braf-mutant mouse model of papillary thyroid cancer. Proc Natl Acad Sci U S A 111(16):E1600-9PubMed: 24711431 MGI: J:208854

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Genetics

Tg(TPO-cre/ERT2)1139Tyj

Allele Symbol: Tg(TPO-cre/ERT2)1139Tyj

Allele Name	transgene insertion 1139, Tyler Jacks
Allele Type	Transgenic (Recombinase-expressing, Inducible)
Allele Synonym(s)	TPOCreER
Gene Symbol and Name	Tg(TPO-cre/ERT2)1139Tyj, transgene insertion 1139, Tyler Jacks
Gene Synonym(s)
Promoter	TPO, thyroid peroxidase, human
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre-ERT2 fusion protein is expressed under direction of the human TPO promoter in the thyroid.
Strain of Origin	C57BL/6NTac
Chromosome	UN
Molecular Note	A construct with the human thyroid peroxidase promoter (TPO) and an inducible cre/estrogen receptor ligand binding domain fusion (cre/ERT2) was used to generate transgenic founders. Two founders were obtained and one (#1139) was used for further analyses.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
Internal/Organ Research
- Thyroid Defects

Mammalian Phenotype Terms by Genotype

References

McFadden DG; Vernon A; Santiago PM; Martinez-McFaline R; Bhutkar A; Crowley DM; McMahon M; Sadow PM; Jacks T. 2014. p53 constrains progression to anaplastic thyroid carcinoma in a Braf-mutant mouse model of papillary thyroid cancer. Proc Natl Acad Sci U S A 111(16):E1600-9PubMed: 24711431 MGI: J:208854

Additional - Tg(TPO-cre/ERT2)1139Tyj related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Generic Cre Probe
- QPCR:Generic Cre Quantitative PCR
- Probe:Trp53 Probe
- Standard PCR:Generic Cre
- Standard PCR:Generic Cre Melt Curve Analysis
- Standard PCR:Tg(TPO-cre/ERT2)1139Tyj
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Noncarrier x Hemizygote
- Hemizygote x Noncarrier
Citation
When using the TPO-CreER mouse strain in a publication, please cite the originating article(s) and include JAX stock #026512 in your Materials and Methods section.

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Additional Use Restrictions Apply

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Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:TPO-CreER

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tg(TPO-cre/ERT2)1139Tyj

Allele Symbol: Tg(TPO-cre/ERT2)1139Tyj

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(TPO-cre/ERT2)1139Tyj related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Additional Use Restrictions Apply

Licensing Information