Akt2flox/flox floxed mice may be useful in applications related to the regulation of lipid metabolism and glucose homeostasis.
Morris J. Birnbaum, Un of Pennsylvania Schl of Medicine
Akt2flox/flox mutant mice possess loxP sites flanking exons 4-5 of the thymoma viral proto-oncogene 2 (Akt2) gene. Akt2 is a serine-threonine protein kinase that functions downstream of the insulin receptor in insulin target tissues, such as liver, muscle, and adipose tissue where it acts to regulate lipid metabolism and glucose homeostasis. When bred to mice that express Cre recombinase, the resulting offspring will have exons 4-5 deleted in cre-expressing tissues.
For example, when bred to mice expressing Cre recombinase in the liver (see Stock No. 003574), resulting mice exhibit reduced liver triglyceride levels when fed a high fat diet.
Mice that are homozygous for this allele are viable and fertile.
A targeting vector was designed to insert a loxP site upstream of exon 4, and a second loxP site followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 5 of the thymoma viral proto-oncogene 2 (Akt2) gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred to C57BL/6J mice for at least 10 generations. Offspring were bred with B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ transgenic mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Akt2flox/flox mice were further backcrossed to C57BL/6J mice for 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.2, Morris J Birnbaum|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Akt2flox; Akt2lox; Akt2loxP|
|Gene Symbol and Name||Akt2, thymoma viral proto-oncogene 2|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||An frt-flanked PGK-neomycin resistance cassette was inserted into intron 5, and loxP sites were inserted flanking exons 4 and 5. Flp-mediated recombination removed the neo cassette leaving exons 4 and 5 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129-Akt2tm1.2Mbb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026475 in your Materials and Methods section.