Overview

Akt1^loxP/loxP floxed mice may be useful for generating conditional mutations in applications related to the study of energy expenditure and metabolism.

Donating Investigator

Morris J. Birnbaum, Un of Pennsylvania Schl of Medicine

Genetic overview

Genetic Background	Generation

Akt1^tm2.2Mbb

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Akt1	thymoma viral proto-oncogene 1

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Research Applications

Metabolism Research
Research Tools
Diabetes and Obesity Research
Cell Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Akt1^loxP/loxP floxed mutant mice possess loxP sites flanking exons 3-4 of the thymoma viral proto-oncogene 1 (Akt1) gene. Akt1 is a serine-threonine protein kinase that functions downstream of phosphotidylinosital 3-kinase in regulating cell survival, insulin signaling, angiogenesis and tumor formation. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-4 deleted in cre-expressing tissues.

For example, when crossed to a strain with germ line Cre recombinase expression (see Stock No. 003724), resulting Akt1^-/- mice are protected from diet-induced obesity and its associated insulin resistance. They have reduced adiposity after high fat diet challenge with elevated oxygen consumption and heat production. They have significantly lower body weight than their wild-type littermates, with heterozygous KO mice displaying an intermediate phenotype.

Mice that are homozygous for this allele are viable and fertile.

Development

A targeting vector was designed to insert a single loxP site upstream of exon 3, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 4 of the thymoma viral proto-oncogene 1 (Akt1) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. Offspring were bred with mice expressing EIIa-cre to remove the floxed neo cassette. Resulting mice contained multiple gene rearrangments; intact floxed-exons 3-4, intact floxed-neo cassette, or excision of exons and the neo cassette. Progeny containing only the floxed-exons were bred to C57BL/6J mice for at least 10 generations to establish a colony of Akt1^loxP/loxP mice. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Wan M; Easton RM; Gleason CE; Monks BR; Ueki K; Kahn CR; Birnbaum MJ. 2012. Loss of Akt1 in mice increases energy expenditure and protects against diet-induced obesity. Mol Cell Biol 32(1):96-106PubMed: 22037765 MGI: J:182721

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Genetics

Akt1^tm2.2Mbb

Allele Symbol: Akt1^tm2.2Mbb

Allele Name	targeted mutation 2.2, Morris J Birnbaum
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Akt1^tm2.2Mbb; targeted mutation 2.2, Morris J Birnbaum
Gene Symbol and Name	Akt1, thymoma viral proto-oncogene 1
Gene Synonym(s)	RAC; AKT; PRKBA; PKB; PKB/Akt; PKBalpha; CWS6; RAC-ALPHA; PKB-ALPHA; Akt
Strain of Origin	129S4/SvJae
Chromosome	12
Molecular Note	A targeting vector was designed to insert a single loxP site upstream of exon 3, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 4 of the gene. Cre-mediated recombination removed the floxed neo cassette.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Metabolism Research
- Lipid Metabolism
Research Tools
- Metabolism Research
- Cre-lox System
  - loxP-flanked Sequences
- Diabetes and Obesity Research
  - loxP
Diabetes and Obesity Research
Cell Biology Research
- Signal Transduction

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Akt1^tm2.2Mbb/Akt1^tm2.2Mbb
involves: 129S4/SvJae * C57BL/6 * FVB/N

normal phenotype

no abnormal phenotype detected
- mice are viable and fertile

References

Wan M; Easton RM; Gleason CE; Monks BR; Ueki K; Kahn CR; Birnbaum MJ. 2012. Loss of Akt1 in mice increases energy expenditure and protects against diet-induced obesity. Mol Cell Biol 32(1):96-106PubMed: 22037765 MGI: J:182721

Additional - Akt1^tm2.2Mbb related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Akt1^tm2.2Mbb
- Standard PCR:Akt1^tm2.2Mbb
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygotes may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the B6.129S4(FVB)-Akt1^tm2.2Mbb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026474 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Akt1<tm2.2Mbb>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6.129S4(FVB)-Akt1<tm2.2Mbb>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S4(FVB)-Akt1<tm2.2Mbb>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S4(FVB)-Akt1<tm2.2Mbb>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.129S4(FVB)-Akt1<tm2.2Mbb>/J Frozen Embryo	$3373.50

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Akt1tm2.2Mbb

Allele Symbol: Akt1tm2.2Mbb

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Akt1tm2.2Mbb/Akt1tm2.2Mbbinvolves: 129S4/SvJae * C57BL/6 * FVB/N

normal phenotype

References

Additional - Akt1tm2.2Mbb related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Akt1^tm2.2Mbb

Allele Symbol: Akt1^tm2.2Mbb

Genotype: Akt1^tm2.2Mbb/Akt1^tm2.2Mbb
involves: 129S4/SvJae * C57BL/6 * FVB/N

Additional - Akt1^tm2.2Mbb related