STOCK Tg(tetO-Ago2)30Mnpy/J

Stock number: 026398
Research areas: Research Tools

Description

Expression of Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO). This strain may be useful in studies of RNA interference and the miRNA pathway.

Detailed description

The Ago2 gene encodes a part of the RNA-induced silencing complex (RISC) and is involved in RNA-mediated gene silencing. Expression of FLAG/HA-tagged Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic teto-Ago2 strain, founder line 30. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA), expression of AGO2 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (January 2018).

When crossed with an Ins-rtTA strain with the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter (e.g. Stock No. 008250), double transgenic “dox-Ago2” mice treated with doxycycline show increased Ago2 protein levels in islet cells and after glucose challenge exhibit temporary glucose intolerance.

Development

Mouse FLAG/HA-tagged Ago2, argonaute RISC catalytic subunit 2, cDNA was placed under the control of the tetO, or TRE2, tetracycline-responsive regulatory element, promoter. The TRE2 (Tet-responsive P_hCMV*-1) promoter, from the Clontech pTRE-2 vector, contains the Tet response element (TRE), which carries seven copies of the 42 bp tet operator sequence, tetO, just upstream of a minimal cytomegalovirus promoter. This strain was created on an unknown genetic background. Founder line 30 was subsequently established. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(tetO-Ago2)30Mnpy
Name: transgene insertion 30, Matthew N Poy
MGI accession ID: MGI:5585175
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-Ago2)30Mnpy
Marker name: transgene insertion 30, Matthew N Poy
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: Ago2,argonaute RISC catalytic subunit 2,mouse, laboratory; HA tag,HA tag,
Site of expression: When mated to a mutant strain expressing rtTA, expression of AGO2 protein may be regulated with tetracycline or its analog doxycycline in tissues where rtTA is expressed.
Molecular note: The tetracycline response element drives inducible expression of the mouse cDNA.