The Ago2 gene encodes a part of the RNA-induced silencing complex (RISC) and is involved in RNA-mediated gene silencing. 
Expression of FLAG/HA-tagged Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic teto-Ago2 strain, founder line 30. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA), expression of AGO2 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (January 2018).
When crossed with an Ins-rtTA strain with the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter (e.g. Stock No. 008250), double transgenic “dox-Ago2” mice treated with doxycycline show increased Ago2 protein levels in islet cells and after glucose challenge exhibit temporary glucose intolerance.

Expression of Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO). This strain may be useful in studies of RNA interference and the miRNA pathway.

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