Expression of Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO). This strain may be useful in studies of RNA interference and the miRNA pathway.
Matthew N. Poy, Max Delbrueck Center for Molecular Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
The Ago2 gene encodes a part of the RNA-induced silencing complex (RISC) and is involved in RNA-mediated gene silencing.
Expression of FLAG/HA-tagged Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic teto-Ago2 strain, founder line 30. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA), expression of AGO2 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (January 2018).
When crossed with an Ins-rtTA strain with the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter (e.g. Stock No. 008250), double transgenic “dox-Ago2” mice treated with doxycycline show increased Ago2 protein levels in islet cells and after glucose challenge exhibit temporary glucose intolerance.
Mouse FLAG/HA-tagged Ago2, argonaute RISC catalytic subunit 2, cDNA was placed under the control of the tetO, or TRE2, tetracycline-responsive regulatory element, promoter. The TRE2 (Tet-responsive PhCMV*-1) promoter, from the Clontech pTRE-2 vector, contains the Tet response element (TRE), which carries seven copies of the 42 bp tet operator sequence, tetO, just upstream of a minimal cytomegalovirus promoter.
This strain was created on an unknown genetic background. Founder line 30 was subsequently established.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | Ago2, argonaute RISC catalytic subunit 2, mouse, laboratory |
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Expressed Gene | HA tag, HA tag, |
Site of Expression | When mated to a mutant strain expressing rtTA, expression of AGO2 protein may be regulated with tetracycline or its analog doxycycline in tissues where rtTA is expressed. |
Allele Name | transgene insertion 30, Matthew N Poy |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | Ago2-tetO |
Gene Symbol and Name | Tg(tetO-Ago2)30Mnpy, transgene insertion 30, Matthew N Poy |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Ago2, argonaute RISC catalytic subunit 2, mouse, laboratory |
Expressed Gene | HA tag, HA tag, |
Site of Expression | When mated to a mutant strain expressing rtTA, expression of AGO2 protein may be regulated with tetracycline or its analog doxycycline in tissues where rtTA is expressed. |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | The tetracycline response element drives inducible expression of the mouse cDNA. |
Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (January 2018).
When using the STOCK Tg(tetO-Ago2)30Mnpy/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026398 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for Tg(tetO-Ago2)30Mnpy |
Frozen Mouse Embryo | STOCK Tg(tetO-Ago2)30Mnpy/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(tetO-Ago2)30Mnpy/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(tetO-Ago2)30Mnpy/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(tetO-Ago2)30Mnpy/J | $3373.50 |
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