The Ago2 gene encodes a part of the RNA-induced silencing complex (RISC) and is involved in RNA-mediated gene silencing. 
Expression of FLAG/HA-tagged Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic teto-Ago2 strain, founder line 30. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA), expression of AGO2 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (January 2018).
When crossed with an Ins-rtTA strain with the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter (e.g. Stock No. 008250), double transgenic “dox-Ago2” mice treated with doxycycline show increased Ago2 protein levels in islet cells and after glucose challenge exhibit temporary glucose intolerance.

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Expression of Ago2 (argonaute RISC catalytic subunit 2) is regulated by a tetracycline operator (tetO). This strain may be useful in studies of RNA interference and the miRNA pathway.

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