These ROSA26-LRRK2 (R1441C) mice allow cre-inducible expression of the familial Parkinson's disease-associated R1441C missense mutant form of LRRK2, and may be useful for studying Parkinson's disease pathogenesis and neurodegeneration.
Darren J Moore, Van Andel Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These mice carry a targeted mutation in which a floxed STOP cassette and mutant human LRRK2 (R1441C) sequence was inserted into the endogenous Gt(ROSA)26Sor locus. The R1441C mutation occurs in the 'Ras of complex proteins' (ROC) domain of the leucine-rich repeat kinase 2 protein, reducing GTPase activity. Homozygotes are viable and fertile.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Of note, the ROSA26-LRRK2 (R1441C) strain is also available on the original STOCK genetic background(Stock No. 022793).
A targeting vector containing a SV40 splice acceptor, a loxP-flanked STOP cassette (PGK-neo and triple SV40 transcriptional termination sequence), a full-length human leucine-rich repeat kinase 2 cDNA bearing a familial Parkinson's disease-linked missense mutation R1441C, and a bovine polyadenylation signal was utilized in the generation of this strain. The construct was inserted into the Gt(ROSA)26Sor locus via electroporation into 129/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6J mice to generate the mutant colony. The donating investigator reported that mice were backcrossed to C57BL/6J for at least 5 generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least 5 generations.
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
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Site of Expression |
Allele Name | targeted mutation 1, Darren Moore |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | R26-LRRK2 |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | The targeting vector contains a SV40 splice acceptor, a loxP-flanked STOP cassette (PGK-neo and triple SV40 transcriptional termination sequence), a full-length human leucine-rich repeat kinase 2 cDNA bearing a familial Parkinson's disease-linked missense mutation R1441C, and a bovine polyadenylation signal. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.Cg-Gt(ROSA)26Sortm1(LRRK2*R1441C)Djmo/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026293 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gt(ROSA)26Sor<tm1(LRRK2*R1441C)Djmo> |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(LRRK2*R1441C)Djmo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(LRRK2*R1441C)Djmo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(LRRK2*R1441C)Djmo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(LRRK2*R1441C)Djmo>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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