Lmnaflox/flox mice possess loxP sites flanking exon 2 of the lamin A gene, making them useful for studying the role of Lamin-A in specific cell types, and its influence on the progression of human diseases.
Yixian Zheng, Carnegie Institution
Lmnaflox/flox mutant mice possess loxP sites flanking exon 2 of the lamin A (Lmna) gene. Lamin-A is part of the nuclear lamina seen in increasing levels of expression as development progresses. It is required for normal development of peripheral nervous system and skeletal muscle and plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. Since different Lamin-A isoforms exist, this strain was developed to produce offspring that lack the majority of Lamin-A isoforms.
A targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked puromycin resistance (puro) cassette followed by a second loxP site, downstream of exon 2 of the lamin A (Lmna) gene. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with CD1 females. Offspring were bred to B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 003800) to delete the puro cassette, and progeny were crossed to remove the Flp-expressing transgene. The resulting Lmnaflox/flox colony was maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.1, Yixian Zheng|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Lmna, lamin A|
|Strain of Origin||(C57BL/6 x 129S4/SvJae)F1|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked puromycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Lmnatm1.1Yxz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026284 in your Materials and Methods section.