STOCK Lmna^tm1.1Yxz/J

Stock number: 026284
Product name: Lmna^flox
Research areas: Cell Biology Research, Developmental Biology Research, Neurobiology Research, Research Tools

Description

Lmna^flox/flox mice possess loxP sites flanking exon 2 of the lamin A gene, making them useful for studying the role of Lamin-A in specific cell types, and its influence on the progression of human diseases.

Detailed description

Lmna^flox/flox mutant mice possess loxP sites flanking exon 2 of the lamin A (Lmna) gene. Lamin-A is part of the nuclear lamina seen in increasing levels of expression as development progresses. It is required for normal development of peripheral nervous system and skeletal muscle and plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. Since different Lamin-A isoforms exist, this strain was developed to produce offspring that lack the majority of Lamin-A isoforms.

Development

A targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked puromycin resistance (puro) cassette followed by a second loxP site, downstream of exon 2 of the lamin A (Lmna) gene. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with CD1 females. Offspring were bred to B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 003800) to delete the puro cassette, and progeny were crossed to remove the Flp-expressing transgene. The resulting Lmna^flox/flox colony was maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Lmna^tm1.1Yxz
Name: targeted mutation 1.1, Yixian Zheng
MGI accession ID: MGI:5575529
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Lmna
Marker name: lamin A
Chromosome: 3
Strain of origin: (C57BL/6 x 129S4/SvJae)F1
Molecular note: A loxP site was inserted upstream of exon 2. An FRT-flanked puromycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed.