Overview

These TRE-mUbe3a_v2[884] transgenic mice allow Tet-Off/Tet-On expression of a FLAG-tagged Ube3a splice variant 2 protein, in addition to normal expression of endogenous Ube3a. These mice are useful to study temporally-controlled, tissue-specific Ube3a variant 2 overexpression as a model for maternal 15q11-13 duplication (dup15) and triplication (isodicentric extranumerary chromosome, idic15); both are associated with human autism spectrum disorder.

Donating Investigator

Scott V Dindot, Texas A&M University

Genetic overview

Genetic Background	Generation

Tg(tetO-Ube3a*2)884Svd

Allele Type
Transgenic (Inducible, Inserted expressed sequence)

View Genetics

Research Applications

Neurobiology Research
Developmental Biology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

The TRE-Ube3av2 transgene has a modified Tet response element (TRE or tetO) upstream of a cDNA sequence encoding a functional, full-length mouse ubiquitin protein ligase E3A transcript variant 2 protein with N-terminal FLAG tag (FLAG-Ube3a_v2). Transgenic mice from founder line 884 (TRE-mUbe3a_v2[884]) are described here.

When these TRE-mUbe3a_v2[884] transgenic mice are bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), FLAG-Ube3a_v2 expression in the resulting double mutant offspring can be regulated with tetracycline or its analog doxycycline (dox). As designed, TRE-mUbe3a_v2[884] transgenic mice have no reported levels of FLAG-Ube3a_v2 expression in absence of tTA. When TRE-mUbe3a_v2[884] transgenic mice are bred to Camk2a-tTA transgenic mice (see Stock Nos. 003010 / 007004), the resulting CaMKIIα-mUbe3a_v2[884] double transgenic mice have FLAG-Ube3a_v2 expression in forebrain neurons.

TRE-mUbe3a_v2[884] transgenic mice have normal expression of endogenous Ube3a. Importantly, the endogenous Ube3a gene is only expressed from the maternal chromosome in neurons; a result of paternal imprinting (the antisense transcript functions to silence paternal expression in brain). Because the TRE-Ube3av2 transgene lacks the transcription initiation site of the antisense transcript, expression of FLAG-Ube3a_v2 is independent of parent-of-origin or sex of the animal.

Hemizygous TRE-mUbe3a_v2[884] transgenic mice are viable and fertile. The donating investigator has not tried to generate homozygous mice to date (December 2014).

Development

The TRE-Ube3av2 transgene was designed in the laboratory of Dr. Scott V. Dindot (Texas A&M University). This transgene has (from 5' to 3') a modified Tet response element (TRE or tetO; described below), a mouse ubiquitin protein ligase E3A (Ube3a) cDNA sequence encoding transcript variant 2 (NM_011668.2), and an SV40 polyA signal. The Ube3a sequence has a FLAG epitope tag attached to its 5' end. The ~3.5 kbp TRE-Ube3av2 transgene was microinjected into FVB/N embryos. Transgenic founder animals were identified by genotyping, and then bred to FVB/NJ. TRE-mUbe3a_v2 founder mice were subsequently bred to FVB/NJ inbred mice. Mice from founder line 884 (TRE-mUbe3a_v2[884]) were propagated and maintained by breeding to FVB/NJ inbred mice and/or wildtype (noncarrier) siblings for several generations. Albino males were sent to The Jackson Laboratory Repository in 2014. Upon arrival, sperm was cryopreserved. To establish our living colony, an aliquot of the frozen sperm was used to fertilize oocytes from FVB/NJ inbred females (Stock No. 001800).

The TRE promoter used here is Tet-responsive P_tight; containing a modified Tet response element (TRE_mod) composed of seven direct 36 bp repeats (each containing the 19-bp tet operator sequence [tetO]). TRE_mod is just upstream of a minimal human cytomegalovirus promoter (P_minCMVΔ), which lacks the enhancer that is part of the complete CMV promoter. Consequently, P_tight is silent in the absence of binding of TetR or rTetR to the tetO sequences.

Expression Data

Expressed Gene	Ube3a, ubiquitin protein ligase E3A, mouse, laboratory
Site of Expression	Expression of FLAG-tagged Ube3a can be regulated with tetracycline or its analog doxycycline when combined with a mutant carrying tTA or rtTA.

Control Suggestions

Noncarrier

Approximate Controls

001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Genetics

Tg(tetO-Ube3a*2)884Svd

Allele Symbol: Tg(tetO-Ube3a*2)884Svd

Allele Name	transgene insertion 884, Scott Dindot
Allele Type	Transgenic (Inducible, Inserted expressed sequence)
Allele Synonym(s)	transgene insertion 884, Scott Dindot; Tg(tetO-Ube3a*2)884Svd
Gene Symbol and Name	Tg(tetO-Ube3a*2)884Svd, transgene insertion 884, Scott Dindot
Gene Synonym(s)
Promoter	tetO, tet operator,
Expressed Gene	Ube3a, ubiquitin protein ligase E3A, mouse, laboratory
Site of Expression	Expression of FLAG-tagged Ube3a can be regulated with tetracycline or its analog doxycycline when combined with a mutant carrying tTA or rtTA.
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	This transgene has (from 5' to 3') a modified Tet response element (TRE or tetO), a mouse ubiquitin protein ligase E3A (Ube3a) cDNA sequence encoding transcript variant 2 (NM_011668.2), and an SV40 polyA signal. The Ube3a sequence has a FLAG epitope tag attached to its 5' end.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Neurodevelopmental Defects
  - Autism
- Tet Expression System
  - tTA/rtTA Responsive Strains
Developmental Biology Research
- Imprinting
- Neurodevelopmental Defects
Research Tools
- Neurobiology Research
  - Tetop Tet System
- Tet Expression Systems
  - tTA/rtTA Responsive Strains
- Genetics Research
  - Mutagenesis and Transgenesis: Tetop Tet System

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(tetO-Ube3a*2)884Svd related

Technical Support

Contact Technical Support

Genotyping Protocols
- High Resolution Melting: Tg(tetO-Ube3a*2)884Svd-V2 and V3
- MELT: Tg(tetO-Ube3a*2)884Svd
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining our live colony, hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to FVB/NJ inbred mice (Stock No. 001800). The donating investigator has not attempted to breed homozygous animals to date (December 2014).
- Additional Breeding and Husbandry Support
Citation
When using the FVB/N-Tg(tetO-Ube3a*2)884Svd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026279 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or non carrier for Tg(tetO-Ube3a*2)884Svd

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Tg(tetO-Ube3a*2)884Svd

Allele Symbol: Tg(tetO-Ube3a*2)884Svd

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(tetO-Ube3a*2)884Svd related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability