B6.129S2(B10)-Il3^tm1Tyb/J

Stock number: 026277
Research areas: Apoptosis Research, Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

IL-3^-/- mice may be useful for studying the growth and survival of hematopoietic cell lineages.

Detailed description

In this strain, a frameshift mutation was placed into exon 1 and a neomycin resistance (neo) cassette replaces part of exon 3 of the interleukin 3 (Il3) gene, abolishing gene expression. IL-3 is a cytokine that promotes the survival, proliferation, and differentiation of hematopoietic progenitors and mature hematopoietic cells. Homozygous mice are viable and fertile. Bone marrow derived mast cells (BMMC) from IL-3 deficient mice exhibit decreased survival when induced with IgE. Expression of anti-apoptotic proteins, Bcl-xL and Bcl-2 are decreased after IgE-induction. When colony stimulating factor 2 receptor, beta (Csf2rd) is knocked out of these mice, double mutants exhibit a lack of eosinophilic response to parasites, and show a pulmonary alveolar proteinosis-like disease.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these C57BL/6J-congenic mice could vary from that originally described on a C57BL/10ScSnJ genetic background. We may modify the strain description if necessary as published results become available.

Development

A targeting vector was designed insert a frameshift mutation at the HindIII site in exon 1 and to replace a 463bp fragment exon 3 of the interleukin 3 (Il3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6JNimr females. These mice were bred to C57BL/10ScSnJNimr mice for at least 10 generations prior to arrival at The Jackson Laboratory Repository (as Stock No. 023816). Upon arrival, some mice were bred to C57BL/6J inbred mice (Stock No. 000664) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6J-congenic strain (Stock No. 026277).

Allele

Symbol: Il3^tm1Tyb
Name: targeted mutation 1, Victor L J Tybulewicz
MGI accession ID: MGI:3586300
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il3
Marker name: interleukin 3
Chromosome: 11
Strain of origin: 129S2/SvPas
Molecular note: As a result of a frameshift mutation in exon 1 and the deletion of exon 2, this allele lacks functional protein.