IL-3-/- mice may be useful for studying the growth and survival of hematopoietic cell lineages.
Victor L.J. Tybulewicz, MRC National Institute for Medical Research
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il3 | interleukin 3 |
In this strain, a frameshift mutation was placed into exon 1 and a neomycin resistance (neo) cassette replaces part of exon 3 of the interleukin 3 (Il3) gene, abolishing gene expression. IL-3 is a cytokine that promotes the survival, proliferation, and differentiation of hematopoietic progenitors and mature hematopoietic cells. Homozygous mice are viable and fertile. Bone marrow derived mast cells (BMMC) from IL-3 deficient mice exhibit decreased survival when induced with IgE. Expression of anti-apoptotic proteins, Bcl-xL and Bcl-2 are decreased after IgE-induction. When colony stimulating factor 2 receptor, beta (Csf2rd) is knocked out of these mice, double mutants exhibit a lack of eosinophilic response to parasites, and show a pulmonary alveolar proteinosis-like disease.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these C57BL/6J-congenic mice could vary from that originally described on a C57BL/10ScSnJ genetic background. We may modify the strain description if necessary as published results become available.
A targeting vector was designed insert a frameshift mutation at the HindIII site in exon 1 and to replace a 463bp fragment exon 3 of the interleukin 3 (Il3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6JNimr females. These mice were bred to C57BL/10ScSnJNimr mice for at least 10 generations prior to arrival at The Jackson Laboratory Repository (as Stock No. 023816). Upon arrival, some mice were bred to C57BL/6J inbred mice (Stock No. 000664) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6J-congenic strain (Stock No. 026277).
Allele Name | targeted mutation 1, Victor L J Tybulewicz |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Il3tm1Mur |
Gene Symbol and Name | Il3, interleukin 3 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 11 |
Molecular Note | As a result of a frameshift mutation in exon 1 and the deletion of exon 2, this allele lacks functional protein. |
Mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for several generations using a marker-assisted, speed congenic approach to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
When using the B6.129S2(B10)-Il3tm1Tyb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026277 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Il3<tm1Tyb> |
Frozen Mouse Embryo | B6.129S2(B10)-Il3<tm1Tyb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2(B10)-Il3<tm1Tyb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2(B10)-Il3<tm1Tyb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S2(B10)-Il3<tm1Tyb>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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