These transgenic mice express reporter lacZ from the proximal -497 bp rat Tnnt2 promoter. This confers cardiac-specific expression in vivo and faithfully recapitulates endogenous expression during development.
Jim Jung-Ching Lin, University of Iowa
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
These mice express a lacZ reporter from the proximal -497 bp Tnnt2 promoter segment derived from rat. LacZ is specifically expressed in the paired cardiogenic mesoderm of day 7.5 postcoitum (p.c.) embryos and in the looping heart tube of day 8 p.c. embryo myocardial cells. As development proceeds, lacZ expression is distributed uniformly throughout both atria and ventricles. By day 10.5 p.c., a low level of lacZ expression starts to be detected in developing somites. At fetal stages, lacZ expression is also detected in developing skeletal muscles, such as intercostal muscles; brachial muscles; muscles associated with metacarpal bones, metatarsal bones, and lower legs; and muscles surrounding the developing vertebrae. The level of transgene expression in the developing skeletal muscle is much lower than that seen in the developing heart. In the adult, lacZ is expressed only in the heart, and not in other tissues tested, including skeletal muscles, liver, spleen, lung, kidney, brain, stomach, and intestine. Recent findings indicate that expression is also seen in cardiomyocytes of pulmonary veins, implicated in the genesis of human cardiac disease and atrial fibrillation.
The construct confers cardiac-specific expression in vivo and faithfully recapitulates endogenous expression during development.
A transgenic vector incorporating the proximal -497 bp segment of the rat Tnnt2 promoter driving lacZ, SV40, and polyA was introduced to B6SJLF1 x B6 embryos. Resultant founder mice were backcrossed to C57BL/6J for at least 2 generations by the donating lab.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression | LacZ is expressed in developing skeletal muscle and heart. In the adult, lacZ is expressed only in the heart. |
Allele Name | transgene insertion 497, Jim J-C Lin |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Tnnt2-lacZ)497Jl, transgene insertion 497, Jim J-C Lin |
Gene Synonym(s) | |
Promoter | Tnnt2, troponin T2, cardiac, mouse, laboratory |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | LacZ is expressed in developing skeletal muscle and heart. In the adult, lacZ is expressed only in the heart. |
Strain of Origin | (C57BL/6 x SJL)F1 x C57BL/6 |
Chromosome | UN |
Molecular Note | The rat Tnnt2 promoter located in the 497 bp immediately 5' of the start codon was used to drive lacZ expression. |
Hemizygotes and homozygotes are viable and fertile.
When using the B6;SJL-Tg(Tnnt2-lacZ)497Jl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026216 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(Tnnt2-lacZ)497Jl |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.