B6.Cg-Prkra^lear-3J/GrsrJ

Stock number: 026195
Product name: little ears 3 Jackson
Research areas: Developmental Biology Research, Reproductive Biology Research, Sensorineural Research

Description

This spontaneous mutant is valuable in the assessment of craniofacial, hearing, and fertility defects related to Prkra and as part of a phenotypically diverse allelic series.

Detailed description

Mice homozygous for the recessive little ear 3 Jackson mutation can be recognized by 2 weeks of age by their smaller ear pinnae. They also have a smaller overall body size, are infertile, and at eight weeks of age were found to have a severe hearing deficit. This is a distinct phenotype from that of the Prkra^tm1Wsmay targeted deletion, which causes complete lethality by embryonic day 3.5.

Development

The little ear 3 Jackson mutation arose spontaneously in the strain B6.B10ScN-Tlr4^lps-del/JthJ strain at The Jackson Laboratory in 2010. The Tlr4^lps-del mutation was bred out of this mutant subline by backcrossing once to C57BL/6J and selectively sibling intercrossing thereafter.

Allele

Symbol: Prkra^lear-3J
Name: little ears 3 Jackson
MGI accession ID: MGI:5461152
Generation method: Spontaneous
Attributes: Not Specified
Marker symbol: Prkra
Marker name: protein kinase, interferon inducible double stranded RNA dependent activator
Chromosome: 2
Strain of origin: B6.B10ScN-Tlr4^lps-del/JthJ
Molecular note: This spontaneous C to T transition at Chromosome 2 position 76,643,218 bp (GRCm38), right after exon 3, likely disrupts splicing of the pre-mRNA.