These floxed mutant mice possess loxP sites flanking the single coding exon of the Cldn4 gene. This strain may be useful for generating conditional mutations in applications related to the study of tight junctions of epithelial cells.
Zea Borok, University of Southern California
The Cldn4 gene encodes for a claudin integral membrane protein that is a component of epithelial cell tight junctions found in epithelium and endothelium.
These mice possess loxP sites on either side of the single coding exon of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the single coding exon deleted in the cre-expressing tissues.
When bred to a strain with germline Cre recombinase expression (see Stock No. 003465 for example), this mutant mouse strain may be useful in studies of acute lung injury.
A targeting vector containing an FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site were inserted downstream of the single coding exon of the targeted gene, and another loxP site was inserted upstream of the single coding exon. This construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts.
Resulting chimeric male animals were crossed to female mice with widespread expression of FLPe recombinase (Stock No. 003946) to remove the FRT flanked selection cassette.
Mice that retained the loxP site flanked single coding exon were then backcrossed to 129S6/SvEvTac for 10 generations. Heterozygotes were crossed to generate homozygotes.
Upon arrival at The Jackson Laboratory, the mice were crossed to 129S1/SvImJ (Stock No. 002448) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Zea Borok|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Cldn4, claudin 4|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A FRT-flanked neomycin selection cassette and a loxP site were inserted downstream of the single coding exon of the targeted gene, and another loxP site was inserted upstream of the single coding exon. FLP mediated recombination removed the FRT-flanked neo cassette leaving the coding exon floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the 129S-Cldn4tm1.1Zboro/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026190 in your Materials and Methods section.