These floxed mutant mice possess loxP sites flanking exon 2 of the Gcm2 gene. This strain may be useful for generating conditional mutations in applications related to the study of parathyroid aplasia and hypoparathyroidism.
Michael A. Levine, The Children's Hospital of Philadelphia
The Gcm2 gene encodes a transcription factor that regulates parathyroid development. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in the male germ line (see Stock No. 003328 for example), this mutant mouse strain may be useful in studies of parathyroid aplasia and
A targeting vector containing a FRT site flanked Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site were inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into unspecified C57BL/6NTac derived embryonic stem (ES) cells.
Correctly targeted ES cells were transiently transfected with a FLP recombinase expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone FLP recombination and no longer retained the cassette but did retain the loxP-flanked exon 2 were injected in blastocysts from B6(Cg)-Tyrc-2J/J (Stock No. 000058). Resulting chimeric male animals were backcrossed to wildtype C57BL/6NTac female mice. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Michael Levine|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Gcm2E2fl; Gcm2tm1Mal2|
|Gene Symbol and Name||Gcm2, glial cells missing homolog 2|
|Strain of Origin||C57BL/6NTac|
|Molecular Note||The targeting vector contains a FRT-flanked neomycin selection cassette and a loxP site inserted downstream of exon 2 of the targeted gene, and another loxP site inserted upstream of exon 2. The neomycin cassette was deleted by transient transfection using flp recombinase.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C57BL/6N-Gcm2tm1.1Malx/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026049 in your Materials and Methods section.