In this transgenic strain, Aldh1l1 promoter-active cells in the brain and spinal cord (the majority being astrocytes) express EGFP. Cre-mediated excision of the floxed EGFP/Stop enables DTA-mediated ablation of these cells.
David H. Rowitch, University of California, San Francisco
Genetic Background | Generation |
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?+pN1F8
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Allele Type |
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Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
These transgenic mice carry loxP-flanked EGFP/Stop and attenuated diptheria toxin A (DTA) chain under the regulatory control of the mouse Aldh1l1 promoter.
In the absence of cre recombinase, Aldh1l1 promoter-active cells in the brain and spinal cord (the majority being astrocytes) express EGFP. When these animals are bred with a cre mouse line, cre-mediated recombination results in tissue-specific deletion of loxP-site flanked EGFP sequence, and subsequently turns on DTA to ablate DTA-expressing cells. This strain is useful for deciphering development and functions of astrocyte subpopulations. Hemizygotes are viable, fertile, and normal in size and do not exhibit physical or behavioral abnormalities.
Intercrosses with Pax3-cre mice (see Stock No. 005549) result in perinatal lethality (10% survivors observed versus 25% expected). The dorsal spinal cord (corresponding to the Pax3
domain) of surviving postnatal day 25 (P25) animals showed atrophy, reduction in the total number of Aldh1l1-expressing cells, loss of neuropil, and congested neurons.
A loxP-EGFP-4XpolyA-loxP-diptheria toxin A (DTA)-polyA cassette was introduced to mouse BAC RP24-510M10 (containing the entire Aldh1l1 gene) just upstream of the start codon. NotI-linearized BAC DNA was injected into B6D2F1 fertilized embryos. The donating laboratory maintained this strain through crosses with outbred Swiss Webster animals.
Expressed Gene | Dta, Diphtheria toxin A chain, |
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Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in the brain and spinal cord. |
Allele Name | transgene insertion D8, David H Rowitch |
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Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Aldh1L1-DTA |
Gene Symbol and Name | Tg(Aldh1l1-EGFP,-DTA)D8Rth, transgene insertion D8, David H Rowitch |
Gene Synonym(s) | |
Promoter | Aldh1l1, aldehyde dehydrogenase 1 family, member L1, mouse, laboratory |
Expressed Gene | Dta, Diphtheria toxin A chain, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in the brain and spinal cord. |
Strain of Origin | (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | A loxP-EGFP-4XpolyA-loxP-diptheria toxin A (DTA)-polyA cassette was introduced to mouse BAC RP24-510M10 (containing the entire Aldh1l1 gene) just upstream of the start codon. |
Hemizygotes are viable and fertile.
When using the STOCK Tg(Aldh1l1-EGFP,-DTA)D8Rth/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026033 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(Aldh1l1-EGFP,-DTA)D8Rth |
Frozen Mouse Embryo | STOCK Tg(Aldh1l1-EGFP -DTA)D8Rth/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Aldh1l1-EGFP -DTA)D8Rth/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Aldh1l1-EGFP -DTA)D8Rth/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Aldh1l1-EGFP -DTA)D8Rth/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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