These Uts2 Apoe double knock-out mice exhibit less severe atherosclerosis and reduced serum cytokine/apdipokine levels when compared to Apoe knockout mice. They are suitable for use in applications related to the study of pathogenesis of and potential treatments for atherosclerosis.
Adel Giaid Schwertani, Montreal General Hospital, RI-MUHC
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Apoe | apolipoprotein E |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Uts2 | urotensin 2 |
The Uts2 gene encodes for a highly conserved vasoconstrictor (vasoactive) peptide. These mice carry a knock out mutation of the Uts2 gene in which half of exon 4 and all of exons 5 and 6 are replaced by lacZ and a Pgk-NEO cassette, as well as a knock out mutation for the Apoe gene. Mice that are homozygous for both targeted mutations are viable and fertile. Double knockout mice exhibit reduced serum cytokine and adipokine levels, less severe aortic atherosclerosis, reduced mean arterial blood pressure, and lower serum very-low-density lipoprotein–cholesterol when compared to the Apoe knock out mice.
For the Uts2 mutant allele, a targeting vector containing IRES sequence, lacZ and a Pgk-NEO cassette was used to disrupt half of exon 4 and all of exons 5 and 6. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6J for 10 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. Heterozygotes were intercrossed to generate homozygotes. The Uts2r knock out mice were then crossed to Apoe knock out mice (see Stock No. 002052). The double mutant mice were intercrossed to produce mice homozygous for both targeted mutations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | AopE(-); apoE-; APOE KO; Apoetm1Un; apoE0; ApoE-KO; EKO; epsilon-; mE-; mEKO |
Gene Symbol and Name | Apoe, apolipoprotein E |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. |
Mutations Made By | Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill |
Allele Name | targeted mutation 1, Adel Giaid Schwertani |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | UII KO |
Gene Symbol and Name | Uts2, urotensin 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 4 |
Molecular Note | A targeting vector containing an IRES sequence, lacZ and a Pgk-NEO cassette was used to disrupt half of exon 4 and all of exons 5 and 6. |
When maintaining a live colony, mice homozygous for both targeted mutations can be bred.
When using the B6.129P2-Uts2tm1Ags Apoetm1Unc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026016 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Uts2<tm1Ags> , Heterozygous for Apoe<tm1Unc> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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