B6.129S1(SJL)-Rcor1^tm2.1Gman/J

Stock number: 025877
Research areas: Research Tools

Description

Exon 4 of the mouse Rorc1 gene is flanked by loxP sites in this conditional mutant allele. Cre-mediated excision of the region creates a null allele.

Detailed description

The corepressor Rcor1 (REST corepressor 1) is essential for murine erythropoiesis. It represses expression of hematopoietic stem cells and myeloid genes.

Exon 4 of the mouse Rcor1 gene are flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region results in a null allele, as determined by Western blot.

Homozygous knockout mice generated through crosses with Meox2-Cre mice (see Stock No. 003755) show a block in fetal erythropoiesis at the proerythroblast stage. Animals die around embryonic day 16.5 due to severe anemia and fetal liver erythropoietic defects. Rcor1 null erythroid progenitors cultured in vitro form myeloid colonies instead of erythroid colonies.

Floxed mice can be bred with Mx1-Cre mice (see Stock No. 003556) to generate embryos in which the expression of Cre is inducible by IFN-α.

Development

A loxP site was introduced to intron 3 and a FRT-PGK-Neo-pA-splice donor-FRT-loxP cassette was placed in intron 4. The mutation was created through homologous recombination in 129S1/Sv-Oca2⁺Tyr⁺Kitl⁺-derived W9.5 embryonic stem (ES) cells. Resultant chimeric mice were crossed with C57BL/6J animals to establish germline transmission. The FRT-flanked neomycin cassette was excised through crosses with a strain expressing FLP recombinase driven by the human ACTB promoter(see Stock No. 003800). Exon 4 remains flanked by loxP sites. Mice were backcrossed to C57BL/6J mice for at least 10 generations by the donating lab.

Allele

Symbol: Rcor1^tm2.1Gman
Name: targeted mutation 2.1, Gail Mandel
MGI accession ID: MGI:5581612
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rcor1
Marker name: REST corepressor 1
Chromosome: 12
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: A loxP site was introduced to intron 3 and a FRT-PGK-Neo-pA-splice donor-FRT-loxP cassette was placed in intron 4. Flpe mediated recombination removed the FRT-flanked neo cassette leaving loxP sites on either side of exon 4.