STOCK Hand1^tm1Eno/AbfiJ

Stock number: 025859
Product name: Hand1^lacZ knock-in/knock-out
Research areas: Cardiovascular Research, Developmental Biology Research, Internal/Organ Research, Research Tools

Description

Hand1^lacZ KO mice express lacZ from the Hand1 promoter making them useful for studying the development of the heart, neural crest (craniofacial, sympathetic neurons, cardiac outflow tract), brachial arch, and yolk sac, as well as vascular development of the left ventricle.

Detailed description

The initiation codon of heart and neural crest derivatives expressed transcript 1 (Hand1) gene was replaced with a β-galactosidase (lacZ) sequence followed by a neomycin resistance cassette in this strain, abolishing gene expression. HAND1 is a basic helix-loop-helix transcription factor expressed in the trophoblast giant cells, yolk sac, cardiac neural crest, lateral mesoderm, septum transversum, early limb buds, and within the myocardial cuff and left ventrical of the developing heart. Heterozygous mice are viable and fertile, while homozygotes die between E8.5 and E9.5 due to defects in heart, extraembryonic mesoderm, and yolk sac development. Lacz staining is detected in extraembryonic mesoderm adjacent to the cardiac crescent, lateral mesoderm, trophectoderm of the ectoplacental cone and extraembryonic mesodermal components of the chorion, amnion, allantois, and visceral yolk sac. Lacz staining is observed in the cardiac outflow tract and the left ventricle of the heart, appearing in cardiac tissue at E8.5.

Development

A targeting vector was designed to replace the initiation codon of the heart and neural crest derivatives expressed transcript 1 (Hand1) gene with a β-galactosidase (lacZ) sequence followed by a neomycin resistance cassette. The construct was electroporated into 129S7/SvEvBrd-Hprt^b-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to Black Swiss females. These mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Hand1^tm1Eno
Name: targeted mutation 1, Eric N Olson
MGI accession ID: MGI:2153824
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Hand1
Marker name: heart and neural crest derivatives expressed 1
Chromosome: 11
Strain of origin: 129S7/SvEvBrd-Hprt1^b-m2
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: LacZ staining in mice carrying this allele is detected in developing lateral mesoderm, trophectoderm of the ectoplacental cone, extraembryonic mesoderm (chorion, amnion, allantois, and visceral yolk sac) and heart (cardiac outflow tract and the left ventricle). Expression was also observed in the mandibular component of the first branchial arch and sympathetic ganglia.
Molecular note: A lacZ reporter gene followed by a neomycin resistance expression cassette was inserted in frame at the position of the initiation codon.