B6;CBA-Tg(Sox10-cre)1Wdr/J

Stock number: 025807
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

Sox10-Cre mice express Cre recombinase in the entire post-migratory post-otic neural crest population during early embryonic development.

Detailed description

Mice hemizygous for the PAC Sox10-cre transgene are viable and fertile. These mice express a nuclear-targeted Cre recombinase directed by the endogenous Sox10 promoter/enhancer regions on the PAC transgene. These mice express cre in the entire post-migratory post-otic neural crest population during early embryonic development and lack expression in mesoderm. When crossed with a strain containing a loxP site-flanked sequence, cre-mediated recombination results in deletion of the flanked sequence in cre-expressing cells of the offspring.

Of note, The Jackson Laboratory live colony in 2017 exhibits white belly spots (not linked to presence of the transgene).

Crispino et al. 2011 PLoS One 6:e23279 reports that Cre recombinase transmission via paternal germline results in early Cre activation in the whole embryo, whereas transmission via maternal germline showed Cre activity only in Sox10 expressing cells.
Furthermore, Luo et al. 2020 Neuron 106:37 Table 1 shows that Sox10-Cre;floxed double mutant males bred to floxed females produced some offspring with germline deletion of the floxed allele. The authors also note that in general, the frequency of recombination in Cre;floxed double mutant germline cells appears to be considerably higher than in zygotes produced by breeding Cre mice to floxed mice. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Sox10-Cre females to floxed males.

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry. This same information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.

Development

A P1-derived artificial chromosome (PAC) (#RP21-529-I6) containing the entire mouse Sox10 (SRY (sex determining region Y)-box 10) gene was modified by targeted mutation to replace the Sox10 gene with a nuclear-targeted Cre recombinase sequence. This modified 120 kb PAC was microinjected into fertilized C57BL6/CBA F1 oocytes. Sox10-cre founders were established and then subsequently maintained by breeding transgenic mice with C57BL6/CBA F1 mice for at least 20 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Sox10-cre)1Wdr
Name: transgene insertion 1, William D Richardson
MGI accession ID: MGI:3586900
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Sox10-cre)1Wdr
Marker name: transgene insertion 1, William D Richardson
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is expressed in the entire post-migratory post-otic neural crest population during early embryonic development.
Molecular note: A nuclear targete cre recombinase sequence replaced the entire coding region of Sox10 in a P1-derived artificial chromosome (PAC ID RP21-529-I6). Several founders were obtained. A representative line was designated line 1.