B6;CBA-Tg(Gsx2-icre)1Kess/J

Stock number: 025806
Product name: Gsh2-iCrepA
Research areas: Neurobiology Research, Research Tools

Description

Gsh2-Cre mice express codon optimized Cre recombinase, iCre, in subcortical precursors and all the neuronal and glial cell progeny.

Detailed description

Mice hemizygous for the PAC Gsh2-cre transgene are viable and fertile. These mice express codon optimized iCre directed by the endogenous Gsx2 (GS homeobox 2) promoter/enhancer regions on the PAC transgene. Cre recombinase expression is seen in precursor cells in the lateral, caudal, and medial ganglionic eminences as well as the septum. When crossed with a strain containing a loxP site-flanked sequence, iCre-mediated recombination results in deletion of the flanked sequence in subcortical precursors and all the neuronal and glial cell progeny.

Development

A P1-derived artificial chromosome (PAC) containing the entire mouse Gsx2 (GS homeobox 2) gene was modified by targeted mutation to harbor codon optimized Cre Recombinase (iCre) sequence, followed by a polyadenylation sequence. A 110kb fragment was isolated from the PAC and was injected into C57BL6/CBA F1 donor oocytes. Resulting offspring were bred to C57BL6/CBA mice for at least 20 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Gsx2-icre)1Kess
Name: transgene insertion 1, Nicoletta Kessaris
MGI accession ID: MGI:3761171
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Gsx2-icre)1Kess
Marker name: transgene insertion 1, Nicoletta Kessaris
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase expression is seen in precursor cells in the lateral, caudal, and medial ganglionic eminences as well as the septum.
Molecular note: A P1 artificial chromosome was created that contained a condon-improved Cre recombinase with a nuclear localization signal fused to the initiation codon. Expression recapitulates endogenous expression in the lateral and or caudal ganglionic eminences with some medial ganglionic eminence expression.