The ACB009 embryonic stem cell line was established using day 3.5 blastocysts from a cross between a hemizygous B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax female (MMRRC #034838/JAX#8730) and a C57BL/6 congenic male homozygous for Mapttm1(EGFP)Klt. This cross resulted in biological replicates that are hemizygous for the transgene (MMRRC#037563) as well as wild type controls (MMRRC#37560, #37561). All lines are heterozygous for Mapttm1(EGFP)Klt. These cell lines have been proven useful in in vitro experiments directed towards differentiation of ESCs to cortical neurons. Cell lines were derived and maintained on mitomycin-C treated mouse embryonic fibroblast feeder cells (MEFs) derived from C57BL/6J embryos. This cell line was not germline tested.

The ACB009 ES cell line was established from a cross between a hemizygous B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas (5XFAD) female (JAX #008730) and a C57BL/6 congenic male homozygous for Mapttm1(EGFP)Klt. This cell line may be useful in in vitro experiments directed towards differentiation of ESCs to cortical neurons expressing AD-relevant mutations.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.