These H2afy or macroH2A1 knockout mice may be useful for studying the involvement of H2A histones in the regulation of gene expression.
John Pehrson, University of Pennsylvania
H2afy or macroH2A1 (H2A histone family, member Y) encodes an alternative H2A histone that includes a unique C-terminal non-histone domain. The macroH2A histones function in a subset of nucleosomes where they are involved in the regulation of gene expression. Homozygous mice are viable, fertile and exhibit no obvious phenotype. When combined with mice carrying H2afy2tm1.1Peh, double mutant mice on the C57BL/6 background breed poorly by either failing to reproduce or exhibiting high rates (29%) of perinatal lethality. On the 129S6 background, double KO mice exhibit abnormally large eyelids (macroblepharon), some eyelid inflammation, and darkened fur on the back (by 6 weeks of age).
This strain may be useful for studying the involvement of H2A histones in the regulation of gene expression.
The H2afy targeting vector was designed to insert a loxP-flanked IRESBgeo cassette upstream of exon 2 and loxP site downstream of exon 2. Exon 2 includes the initiation codon and encodes the first part of the H2A domain. The construct was electroporated into a 129X1/SvJ-derived embryonic stem (ES) cells. Transient cre expression removed the floxed sequence. The resulting chimeric animals were crossed to C57BL/6 for at least 10 generations.
Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, John R Pehrson|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Macroh2a1, macroH2A.1 histone|
|Strain of Origin||129X1/SvJ|
|General Note||ES cell line = 98-2 (Incyte Genomics; "129svJ").|
|Molecular Note||An IRESBgeo cassette was inserted in 1st intron. This cassette was flanked by LoxP sites and a 3rd loxP site was inserted in the 2nd intron. Transient cre expression removed the floxed sequence, including exon 2, which encodes the first part of the H2A domain. Western blot analysis confirmed the absence of protein product.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the macroH2A1 KO mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37472 in your Materials and Methods section.