H2afy or macroH2A1 (H2A histone family, member Y) encodes an alternative H2A histone that includes a unique C-terminal non-histone domain. The macroH2A histones function in a subset of nucleosomes where they are involved in the regulation of gene expression. Homozygous mice are viable, fertile and exhibit no obvious phenotype. When combined with mice carrying H2afy2tm1.1Peh, double mutant mice on the C57BL/6 background breed poorly by either failing to reproduce or exhibiting high rates (29%) of perinatal lethality. On the 129S6 background, double KO mice exhibit abnormally large eyelids (macroblepharon), some eyelid inflammation, and darkened fur on the back (by 6 weeks of age). 


This strain may be useful for studying the involvement of H2A histones in the regulation of gene expression.

These H2afy or macroH2A1 knockout mice may be useful for studying the involvement of H2A histones in the regulation of gene expression.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.