Exons 5 and 6 of the Sec24d gene have been deleted in this Sec24d-Null mutant strain. These mice are useful in studies of embryonic development and endoplasmic reticulem (ER)-Golgi vesicular trafficking.
David Ginsburg, University of Michigan
SEC24 proteins are responsible for selectively recruiting cargo proteins from the endoplasmic reticulum (ER) into COPII vesicles that mediate transport to the Golgi. Sec24d (Sec24 related gene family, member D (S. cerevisiae)) is one of four mammalian Sec24 paralogs which are highly conserved at the C-terminus and variable in the N-terminal region. Mice completely lacking SEC24D die during embryonic development.
Exons 5 and 6 of the Sec24d gene have been deleted in this null allele. Homozygotes are presumed to be embryonic lethal prior to E3.5. Heterozygotes are viable, healthy, and fertile, and without an overt phenotype. It has not been confirmed whether the targeted allele produces any protein.
Embryonic stem (ES) cell clone HEPD0753_7_H04 (EUCOMM ID: LOC383951) was created as part of the Knockout Mouse Project (KOMP). The original gene trapped allele of Sec24d incorporated an FRT-En2 splice acceptor (SA)-IRES-lacZ-SV40 polyadenylation signal (pA)-loxP-PGK-Neo-SV40 polyadenylation signal (pA)-FRT-loxP sequence in intron 2 of the gene and an additional loxP site in intron 3 to create the Sec24d-GT (tm1a(EUCOMM)Hmgu) allele. C57BL/6N-Atm1Brd-derived JM8A3.N1 embryonic stem (ES) cells were used to create the mutation. Chimeras were crossed with B6(Cg)-Tyrc-2J/J (see Stock No. 000058) to achieve germline transmission. The lacZ and neomycin segments were excised with germline Flp recombinase (see Stock No. 005703), leaving exons 5 and 6 flanked by loxP sites. Then animals were crossed with a germline EIIa-Cre strain (see Stock No. 003724) to delete exons 5 and 6. This strain was backcrossed to C57BL/6J for four generations by the donating laboratory. The Tyrc-2J allele was bred out of the line. The donating laboratory observed black and agouti mice in their colony.
|Allele Name||targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Sec24d, Sec24 related gene family, member D (S. cerevisiae)|
|Strain of Origin||C57BL/6N-Atm1Brd|
|Molecular Note||The L1L2_Bact_P cassette was inserted at position 123293030 of Chromosome 3 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 123294426. The critical exon(s) is/are thus flanked by loxP sites. The lacZ and neomycin segments were excised with germline Flp recombinase, leaving exons 5 and 6 flanked by loxP sites. Then animals were crossed with a germline EIIa-Cre strain to delete exons 5 and 6. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.|
Heterozygotes are viable and fertile. Homozygotes are presumed to be embryonic lethal.
When using the B6J.B6N(Cg)-Sec24dtm1d(EUCOMM)Hmgu/DgiJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #025749 in your Materials and Methods section.