These transgenic mice carry the FGFR-HFc inhibitor of FGF signaling, regulated by a tetracycline operator (tetO)/minimal cytomegalovirus (CMV) promoter. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of the dominant-negative FGFR-HFc protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. These mice may be useful for studying FGF signaling in development of the embryo and in the adult mouse.
Dr. Jeffrey Whitsett, Children's Hospital Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
The Fgfr2b gene encodes for a tyrosine protein kinase, fibroblast growth factor receptor, that is required for embryonic patterning, lung development, osteoblast differentiation, and skin development. The soluble chimeric dominant-negative FGFR-HFc construct consists of the extracellular domain of the mouse Fgfr2b, fibroblast growth factor receptor 2, gene and the heavy chain hinge and Fc domain of the mouse immunoglobulin (Igh, immunoglobulin heavy chain complex).
Expression of the FGFR-HFc construct is regulated by a tetracycline operator (tetO)/minimal cytomegalovirus (CMV) promoter, in this transgenic strain. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of the mutant Fgfr2 may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring.
Mice hemizygous for the transgene are viable and fertile.
The Donating Investigator has not attempted to make the strain homozygous.
A transgenic vector containing the soluble chimeric dominant-negative FGFR-HFc construct, which consists of the extracellular domain of the mouse Fgfr2b, fibroblast growth factor receptor 2, gene and the heavy chain hinge and Fc domain of the mouse immunoglobulin (Igh, immunoglobulin heavy chain complex), under control of a tetracycline operator (tetO)/minimal cytomegalovirus (CMV) promoter, was utilized in the construction of this transgenic strain. The construct was microinjected into FVB/N fertilized oocytes. Founder line 1.3 was subsequently established and maintained on the FVB/N background (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Five of the 27 markers throughout the genome were segregating, suggesting an unknown contributing background.
Expressed Gene | Igh, immunoglobulin heavy chain complex, mouse, laboratory |
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Expressed Gene | Fgfr2, fibroblast growth factor receptor 2, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 1.3, Jeffrey A Whitsett |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | (tetO)7-CMV-FGFR-HFc; Tet-sFgfr2b |
Gene Symbol and Name | Tg(tetO-Fgfr2b/Igh)1.3Jaw, transgene insertion 1.3, Jeffrey A Whitsett |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Igh, immunoglobulin heavy chain complex, mouse, laboratory |
Expressed Gene | Fgfr2, fibroblast growth factor receptor 2, mouse, laboratory |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | A transgenic vector containing the soluble chimeric dominant-negative FGFR-HFc construct, which consists of the extracellular domain of the mouse Fgfr2b gene and the heavy chain hinge and Fc domain of the mouse immunoglobulin, under control of a tetracycline operator (tetO)7/minimal cytomegalovirus (CMV) promoter, was utilized in the construction of this transgenic strain. Founder lines 1.3 and 5.9 were generated, it is not known how they compare to each other. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator has not attempted to make the strain homozygous.
When using the STOCK Tg(tetO-Fgfr2b/Igh)1.3Jaw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025672 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(tetO-Fgfr2b/Igh)1.3Jaw |
Frozen Mouse Embryo | STOCK Tg(tetO-Fgfr2b/Igh)1.3Jaw/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(tetO-Fgfr2b/Igh)1.3Jaw/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(tetO-Fgfr2b/Igh)1.3Jaw/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(tetO-Fgfr2b/Igh)1.3Jaw/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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