A transgenic vector containing the soluble chimeric dominant-negative FGFR-HFc construct, which consists of the extracellular domain of the mouse Fgfr2b, fibroblast growth factor receptor 2, gene and the heavy chain hinge and Fc domain of the mouse immunoglobulin (Igh, immunoglobulin heavy chain complex), under control of a tetracycline operator (tetO)/minimal cytomegalovirus (CMV) promoter, was utilized in the construction of this transgenic strain. The construct was microinjected into FVB/N fertilized oocytes. Founder line 1.3 was subsequently established and maintained on the FVB/N background (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Five of the 27 markers throughout the genome were segregating, suggesting an unknown contributing background.
