STOCK Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} Tg(tetO-Fgf10)1Jaw/SpdlJ

Stock number: 025671
Product name: (teto)7CMV-FGF-10
Research areas: Research Tools

Description

In these mutant mice, widespread expression of the Fgf10 gene may be regulated with the tetracycline analog doxycycline (DOX). These mice may be useful in studies of embryonic development and morphogenesis.

Detailed description

The Fgf10 gene encodes the fibroblast growth factor 10 which is important in embryonic epidermal morphogenesis, limb bud formation, and lung and brain development. These mice carry a knock in allele containing the mouse Fgf10 under the control of the tetO, tetracycline-responsive element, and the the Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} allele which allows for Doxyclycine inducible widespread expression of the Fgf10 gene in all tissues. The Donating Investigator reports mice that are homozygous for both the transgene and targeted mutation allele are viable and fertile.

Development

A transgenic insert containing the mouse Fgf10 gene, the 3’ UTR of the bovine growth hormone gene (polyA), under the control of tetO, (teto)7CMV, containing seven direct repeats of the tetracycline operator (tetO) sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter, was injected into FVB/N oocytes. The transgenic mice were crossed with mice carrying the Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy} allele (see Stock No. 005670) and Tg(CMV-cre)1Cgn transgene (see Stock No. 003465), which resulted in the Cre recombinase mediated excision of the floxed STOP cassette in the Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy} allele, creating the widely expressed Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} allele. The mice were then crossed to other various mutant lines on different genetic backgrounds including but not limited to Swiss outbred. The Tg(CMV-cre)1Cgn transgene was bred out of the line to generate this double mutant line. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(tetO-Fgf10)1Jaw
Name: transgene insertion 1, Jeffrey A Whitsett
MGI accession ID: MGI:5581842
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-Fgf10)1Jaw
Marker name: transgene insertion 1, Jeffrey A Whitsett
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: Fgf10,fibroblast growth factor 10,mouse, laboratory
Site of expression: Widespread expression of Fgf10 can be regulated with doxyclycine when combined with a mutation carrying rtTA.
Molecular note: The transgenic insert is comprised of the mouse Fgf10 gene followed by the 3' UTR of the bovine growth hormone gene (polyA), under the control of (teto)₇CMV, which contains seven direct repeats of the tetracycline operator sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter. Founder lines A and B (described in the original reference) were also generated.

Allele

Symbol: Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}
Name: targeted mutation 1.1, Andras Nagy
MGI accession ID: MGI:5286599
Generation method: Targeted
Attributes: Reporter; Transactivator
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Widespread reverse tetracycline transactivator (rtTA)-expression.
Molecular note: A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Cre-mediated recombination removed the floxed neo cassette allowing the ROSA26 promoter to drive expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene.