In these mutant mice, widespread expression of the Fgf10 gene may be regulated with the tetracycline analog doxycycline (DOX). These mice may be useful in studies of embryonic development and morphogenesis.
Dr. Jeffrey Whitsett, Children's Hospital Medical Center
Stijn P. De Langhe, National Jewish Health
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Transactivator) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Allele Type |
---|
Transgenic (Inducible, Inserted expressed sequence) |
The Fgf10 gene encodes the fibroblast growth factor 10 which is important in embryonic epidermal morphogenesis, limb bud formation, and lung and brain development. These mice carry a knock in allele containing the mouse Fgf10 under the control of the tetO, tetracycline-responsive element, and the the Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy allele which allows for Doxyclycine inducible widespread expression of the Fgf10 gene in all tissues. The Donating Investigator reports mice that are homozygous for both the transgene and targeted mutation allele are viable and fertile.
A transgenic insert containing the mouse Fgf10 gene, the 3’ UTR of the bovine growth hormone gene (polyA), under the control of tetO, (teto)7CMV, containing seven direct repeats of the tetracycline operator (tetO) sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter, was injected into FVB/N oocytes. The transgenic mice were crossed with mice carrying the Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy allele (see Stock No. 005670) and Tg(CMV-cre)1Cgn transgene (see Stock No. 003465), which resulted in the Cre recombinase mediated excision of the floxed STOP cassette in the Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy allele, creating the widely expressed Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy allele. The mice were then crossed to other various mutant lines on different genetic backgrounds including but not limited to Swiss outbred. The Tg(CMV-cre)1Cgn transgene was bred out of the line to generate this double mutant line. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | Widespread reverse tetracycline transactivator (rtTA)-expression. |
Expressed Gene | Fgf10, fibroblast growth factor 10, mouse, laboratory |
Site of Expression | Widespread expression of Fgf10 can be regulated with doxyclycine when combined with a mutation carrying rtTA. |
Allele Name | targeted mutation 1.1, Andras Nagy |
---|---|
Allele Type | Targeted (Reporter, Transactivator) |
Allele Synonym(s) | ROSA26rtTA |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Widespread reverse tetracycline transactivator (rtTA)-expression. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 6 |
Molecular Note | A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Cre-mediated recombination removed the floxed neo cassette allowing the ROSA26 promoter to drive expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. |
Allele Name | transgene insertion 1, Jeffrey A Whitsett |
---|---|
Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | (teto)7CMV-FGF-10; Tet-Fgf10 |
Gene Symbol and Name | Tg(tetO-Fgf10)1Jaw, transgene insertion 1, Jeffrey A Whitsett |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Fgf10, fibroblast growth factor 10, mouse, laboratory |
Site of Expression | Widespread expression of Fgf10 can be regulated with doxyclycine when combined with a mutation carrying rtTA. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgenic insert is comprised of the mouse Fgf10 gene followed by the 3' UTR of the bovinegrowth hormone gene (polyA), under the control of (teto)7CMV, which contains seven direct repeats of the tetracycline operator sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter. Founder lines A and B (described in the original reference) were also generated. |
When maintaining a live colony, mice that are hemizygous for transgene and homozygous for the Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy allele may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator reports mice that are homozygous for both the transgene and targeted mutation allele are viable and fertile.
When using the (teto)7CMV-FGF-10 mouse strain in a publication, please cite the originating article(s) and include JAX stock #025671 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1.1(rtTA,EGFP)Nagy>, Hemizygous or Non carrier for Tg(tetO-Fgf10)1Jaw |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1.1(rtTA EGFP)Nagy> Tg(tetO-Fgf10)1Jaw | $2595.00 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1.1(rtTA EGFP)Nagy> Tg(tetO-Fgf10)1Jaw | $2595.00 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1.1(rtTA EGFP)Nagy> Tg(tetO-Fgf10)1Jaw | $3373.50 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1.1(rtTA EGFP)Nagy> Tg(tetO-Fgf10)1Jaw | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.