STOCK Myf6^{tm1.1(cre/ERT2)Lepr}/J

Stock number: 025669
Product name: Myf6^CE
Research areas: Cell Biology Research, Research Tools

Description

These mice express tamoxifen-inducible Cre recombinase under the direction of the Myf6 promoter in muscle.

Detailed description

Myf6^CE mice express a Cre-ERT2 fusion protein from endogenous Myf6 promoter/enhancer elements. MYF6 is a DNA binding protein involved in the development of muscle. Heterozygous mice are viable and fertile. When Myf6^CE mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences.

For example, when bred to B6.129S4-Gt(ROSA)26Sor^tm1Sor/J mice (Stock No. 003474), tamoxifen-inducible cre-mediated recombination results in lacZ staining of somite from E8.75 to E10.75, and in trunk and limb muscles with increasing intensity from E12.75 to E14.75. lacZ staining was evident in the limb muscles of adult animals, and was absent from the heart.

Development

A targeting vector was designed to replace exon 1e of the myogenic factor 6 (Myf6) gene with a CreERT2 (Cre recombinase fused to an estrogen receptor ligand binding domain) coding sequence and a frt-flanked neomycin (neo) resistance cassette. This construct was electroporated into C57BL/6;129sv embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Tg(ACTFLPe)9205Dym/J mice to remove the neo selection cassette. Offspring were then crossed to remove the Flp-expressing transgene. These mice were bred to 129svJ mice for at least 3 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Myf6^{tm1.1(cre/ERT2)Lepr}
Name: targeted mutation 1.1, Christoph Lepper
MGI accession ID: MGI:5620013
Generation method: Targeted
Attributes: Recombinase-expressing; Inducible; Null/Knockout
Synonyms: Myf6^CE
Marker symbol: Myf6
Marker name: myogenic factor 6
Marker synonyms: bHLHc4; CNM3; herculin; MRF4; MRF4AA; myf-6
Chromosome: 10
Strain of origin: C57BL/6 x 129
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Cre-ERT2 fusion protein is expressed from endogenous Myf6 promoter/enhancer elements, which are involved in the development of muscle.
Molecular note: A rabbit beta-globin intron, cre/ERT2 fusion gene, SV40 late polyadenylation signal and FRT-flanked neomycin cassette replaced the coding sequence in exon 1. Flp-mediated recombination removed the selection cassette.