CC10-OVA transgenic mice have expression of a membrane-bound exogenous antigen (ovalbumin with OT-1 and OT-11 epitopes) directed to large and small airway epithelium of the lungs (~50-60% of the airway lining) by the rat Clara cell secretory protein promoter. Transgenic carrier mice are viable and fertile with normal development and no abnormal pathology. Prior to adoptive transfer of cells the lungs remain normal. These mice are useful for modeling antigen-specific, T-cell mediated acute bronchiolitis/pneumonitis as well as lung pathology similar to humans with acute lung transplant rejection and virus-induced lung injury. 
 Lung-specific injury may be induced by adoptive transfer of activated OVA-specific T cells. Specifically, CC10-OVA transgenic mice adoptively transferred with 5x105 activated OT-I-specific cytotoxic T lymphocytes (CTL) die within 6 days due to severe lung injury, while transfer of a lower amount of OT-I cells (1x105) decreases mortality (40% survive). Transfer of 5x105 OT-I cells cultured with low IL-2 resulted in significantly decreased mortality compared to the 5x105 OT-I cultured under high IL-2 conditions. Transfer of 1x105 OT-I cultured with low dose IL-2 was not lethal, but an acute inflammatory response was still evident in the lungs. This inflammatory phenotype resolved in 30 days (and required endogenous ICOS-expressing CD4+ bystander lymphocytes). Similarly-treated Rag1-deficient B6.CC10-OVA mice (B6.CC10-OVA.Rag1-/-) succumb to lethal lung rejection/pneumonitis (as they lack functional CD4+ lymphocytes), and this injury may be attenuated via co-transfer with T regulatory cells.
 The donating investigator reports that breeding transgenic carrier mice together produces homozygous animals that are viable and fertile. In addition, those homozygous mice exhibit no difference in experimental performance compared to transgenic carrier mice produced from transgenic carrier x wildtype (noncarrier) matings.

CC10-OVA transgenic mice express a membrane-bound exogenous antigen (ovalbumin with OT-1 and OT-11 epitopes) directed to large and small airway epithelium of the lungs by the rat Clara cell secretory protein promoter. These mice are useful for modeling antigen-specific, T-cell mediated acute bronchiolitis/pneumonitis as well as lung pathology similar to humans with acute lung transplant rejection and virus-induced lung injury.

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