STOCK Islr2^tm2.1Ddg/J

Stock number: 025615
Research areas: Neurobiology Research, Research Tools

Description

A portion of exon 3 of the mouse Islr2 (Linx) gene is flanked by loxP sites in this targeted mutant strain. Cre excision of the floxed region results in a knockout of the gene.

Detailed description

Islr2 (immunoglobulin superfamily containing leucine-rich repeat 2; also called Linx) is expressed in the peripheral nervous system, prethalamus, lateral ganglionic eminence-derived corridor, corticofugal axons and elsewhere. It binds to thalamocortical projections and promotes their outgrowth guiding them in the ventral forebrain and mediating reciprocal interactions between ascending thalamocortical and descending corticofugal axons in the internal capsule (IC) region.

A portion of exon 3 of the mouse Islr2 gene is flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region results in a knockout allele. Mice deficient in this transmembrane protein exhibit a complete absence of the internal capsule (IC).

Development

A loxP site was introduced to intron 2 and a FRT-PGK-Neo-pA-FRT-loxP cassette was introduced to exon 3 via homologous recombination in 129S6/SvEvTac-derived embryonic stem (ES) cells. Resultant chimeric mice were crossed with C57BL/6-SJL mixed background Flp mice (see Stock No. 003800) to excise the neomycin cassette, leaving a portion of exon 3 flanked by loxP sites. This strain was backcrossed several times to C57BL/6J by the donating laboratory.

Allele

Symbol: Islr2^tm2.1Ddg
Name: targeted mutation 2.1, David D Ginty
MGI accession ID: MGI:5569767
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Islr2
Marker name: immunoglobulin superfamily containing leucine-rich repeat 2
Chromosome: 9
Strain of origin: 129S6/SvEvTac
Molecular note: A loxP site was introduced to intron 2 and a FRT-PGK-Neo-pA-FRT-loxP cassette was introduced to exon 3. Flp-mediated recombination removed the selection cassette and left exon 3 floxed.