STOCK Dlx1^tm1Rth Dlx2^tm1.1Rth/J

Stock number: 025612
Research areas: Neurobiology Research, Research Tools

Description

This conditional mutant strain enables concurrent cre-mediated knockout of the Dlx1 and Dlx2 genes.

Detailed description

Dlx1 (distal-less homeobox 1) and Dlx2 (distal-less homeobox 2) are inversely oriented in close proximity (~10 kb apart) on mouse Chromosome 2. The genes have been implicated in craniofacial, skeletal, and neuronal development processes.

LoxP sites located in intron 1 of each gene enable concurrent cre-mediated excision of the intervening region and knock out both genes.

Development

Dlx1 (distal-less homeobox 1) and Dlx2 (distal-less homeobox 2) are inversely oriented and in close proximity (~10 kb apart) on mouse Chromosome 2. A loxP site was introduced to intron 1 of the Dlx1 gene and an FRT-flanked PGK-neomycin cassette and loxP site were placed in intron 1 of the mouse Dlx2 gene through homologous recombination in an unknown embryonic stem cell line. The PGK-neomycin cassette was excised through crosses with a Gt(ROSA)26Sor-Flp strain. This strain was maintained on a mixed genetic background that includes a 129Sv contribution by the donating investigator.

Allele

Symbol: Dlx2^tm1.1Rth
Name: targeted mutation 1.1, David H Rowitch
MGI accession ID: MGI:5575409
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Dlx2
Marker name: distal-less homeobox 2
Chromosome: 2
Strain of origin: (C57BL/6 x 129S/SvEv)F1
Molecular note: An FRT-flanked PGK-neomycin cassette and loxP site were placed in intron 1. Flp-mediated recombination removed the selection cassette.
General note: This allele was generated in ES cells in conjunction with Dlx1^tm1Rth.

Allele

Symbol: Dlx1^tm1Rth
Name: targeted mutation 1, David H Rowitch
MGI accession ID: MGI:5575408
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Dlx1
Marker name: distal-less homeobox 1
Chromosome: 2
Strain of origin: (C57BL/6 x 129S/SvEv)F1
Molecular note: A loxP site was introduced to intron 1.
General note: This allele was generated in ES cells in conjunction with Dlx2^tm1.1Rth.