This conditional mutant strain enables concurrent cre-mediated knockout of the Dlx1 and Dlx2 genes.
David H. Rowitch, University of California, San Francisco
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed)) | Dlx1 | distal-less homeobox 1 |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed)) | Dlx2 | distal-less homeobox 2 |
Dlx1 (distal-less homeobox 1) and Dlx2 (distal-less homeobox 2) are inversely oriented in close proximity (~10 kb apart) on mouse Chromosome 2. The genes have been implicated in craniofacial, skeletal, and neuronal development processes.
LoxP sites located in intron 1 of each gene enable concurrent cre-mediated excision of the intervening region and knock out both genes.
Dlx1 (distal-less homeobox 1) and Dlx2 (distal-less homeobox 2) are inversely oriented and in close proximity (~10 kb apart) on mouse Chromosome 2. A loxP site was introduced to intron 1 of the Dlx1 gene and an FRT-flanked PGK-neomycin cassette and loxP site were placed in intron 1 of the mouse Dlx2 gene through homologous recombination in an unknown embryonic stem cell line. The PGK-neomycin cassette was excised through crosses with a Gt(ROSA)26Sor-Flp strain. This strain was maintained on a mixed genetic background that includes a 129Sv contribution by the donating investigator.
Allele Name | targeted mutation 1, David H Rowitch |
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Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Dlx1/Dlx2tm1.1Rth |
Gene Symbol and Name | Dlx1, distal-less homeobox 1 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S/SvEv)F1 |
Chromosome | 2 |
General Note | This allele was generated in ES cells in conjunction with Dlx2tm1.1Rth. |
Molecular Note | A loxP site was introduced to intron 1. |
Allele Name | targeted mutation 1.1, David H Rowitch |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Dlx1/Dlx2tm1.1Rth |
Gene Symbol and Name | Dlx2, distal-less homeobox 2 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S/SvEv)F1 |
Chromosome | 2 |
General Note | This allele was generated in ES cells in conjunction with Dlx1tm1Rth. |
Molecular Note | An FRT-flanked PGK-neomycin cassette and loxP site were placed in intron 1. Flp-mediated recombination removed the selection cassette. |
Homozygous and heterozygous mice are viable and fertile.
When using the STOCK Dlx1tm1Rth Dlx2tm1.1Rth/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025612 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dlx1/Dlx2<tm1.1Rth> |
Frozen Mouse Embryo | STOCK Dlx1<tm1Rth> Dlx2<tm1.1Rth>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Dlx1<tm1Rth> Dlx2<tm1.1Rth>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Dlx1<tm1Rth> Dlx2<tm1.1Rth>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Dlx1<tm1Rth> Dlx2<tm1.1Rth>/J Frozen Embryo | $3373.50 |
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