These Ifngr1 knock out mice exhibit increased susceptibility to bacterial infection and may be useful for studies related to innate immunity. Mouse mutants involving this gene have been used in studies of Zika virus pathogenesis.
Robert Schreiber, Washington University School of Medicine
Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (protein) is detected by flow cytometry analysis of spleen, peripheral blood leukocytes or thymus. Homozygous mice exhibit increased susceptibility to Listeria monocytogenes infection. CD8a+ dendritic cells from Ifngr1WU -/- mice infected with Listeria monocytogenes do not produce IL-12.
A targeting vector containing a floxed neomycin resistance cassette was utilized in the construction of this mutant. This selection cassette inserted upstream of exon 3, and another loxP site was inserted downstream of exon 4. This construct was electroporated into C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley derived B6/BLU embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector (pTurbo-cre) to remove the selection cassette. Correctly targeted ES cells in which Cre recombination excised exons 3 and 4 and the selection cassette, were microinjected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were maintained on a C57BL/6NTac background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
|Allele Name||targeted mutation 1.2, Robert D Schreiber|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ifngr1, interferon gamma receptor 1|
|Strain of Origin||C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley|
|Molecular Note||A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed the selection cassette and exons 3 and 4. Flow cytometry confirmed the absence of protein expression in spleen, peripheral blood leukocytes and thymus.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Ifngr1WU KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #025545 in your Materials and Methods section.