C57BL/6N-Ifngr1^tm1.2Rds/J

Stock number: 025545
Product name: Ifngr1WU KO

Description

These Ifngr1 knock out mice exhibit increased susceptibility to bacterial infection and may be useful for studies related to innate immunity. Mouse mutants involving this gene have been used in studies of Zika virus pathogenesis.

Detailed description

Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (protein) is detected by flow cytometry analysis of spleen, peripheral blood leukocytes or thymus. Homozygous mice exhibit increased susceptibility to Listeria monocytogenes infection. CD8a+ dendritic cells from Ifngr1_WU -/- mice infected with Listeria monocytogenes do not produce IL-12.

Development

A targeting vector containing a floxed neomycin resistance cassette was utilized in the construction of this mutant. This selection cassette inserted upstream of exon 3, and another loxP site was inserted downstream of exon 4. This construct was electroporated into C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley derived B6/BLU embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector (pTurbo-cre) to remove the selection cassette. Correctly targeted ES cells in which Cre recombination excised exons 3 and 4 and the selection cassette, were microinjected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were maintained on a C57BL/6NTac background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Allele

Symbol: Ifngr1^tm1.2Rds
Name: targeted mutation 1.2, Robert D Schreiber
MGI accession ID: MGI:5558049
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ifngr1
Marker name: interferon gamma receptor 1
Chromosome: 10
Strain of origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley
Molecular note: A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed the selection cassette and exons 3 and 4. Flow cytometry confirmed the absence of protein expression in spleen, peripheral blood leukocytes and thymus.