These floxed mutant mice possess loxP sites flanking exon 3 of the Paip2b gene. This strain may be useful for generating conditional mutations in applications related to the regulation of mRNA translation and spermiogenesis.
Nahum Sonenberg, McGill University
These mice possess loxP sites on either side of exon 3 of the targeted Paip2b gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. The Paip2b gene encodes a Poly(A) Binding Protein Interacting Protein, which acts as a translational inhibitor.
A targeting vector containing a loxP site flanked NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 3. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone Cre mediated recombination and no longer retained the cassette but did retain the loxP-flanked exon 3 were injected in blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6 mice for 15 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Nahum Sonenberg|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Paip2b, poly(A) binding protein interacting protein 2B|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A floxed neo cassette was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. Cre mediated recombination removes the neo cassette.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129-Paip2btm1.1Nso/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025437 in your Materials and Methods section.