Hhexflox/flox mice may be useful for studying hepatoblast differentiation and intrahepatic bile duct morphogenesis, as well as early lymphoid specification.
Clifford Bogue, Yale University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Hhex | hematopoietically expressed homeobox |
These Hhexflox/flox mutant mice possess loxP sites flanking exons 2-3 of the hematopoietically expressed homeobox (Hhex) gene. HHEX is a transcription factor required for production of all lymphoid lineages and for early development of the liver. Expression is seen in the ventral foregut endoderm at E8.5 and in hepatobiliary tissue during later stages of development. HHEX expression is also evident in adult liver in hepatocytes and biliary epithelial cells in the intrahepatic bile ducts. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.
For example, when bred to mice expressing Cre Recombinase in the hepatic diverticulum, resulting offspring were embryonic lethal and had small and cystic livers, absent gall bladders and extrahepatic bile ducts.
When bred to mice expressing Cre Recombinase in the embryonic liver, resulting mice exhibited irregular development of intrahepatic bile ducts, cystic biliary epithelial cells, and a slow, progressive form of polycystic liver disease in adult mice.
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette followed by a loxP site upstream of exon 2, and a second loxP site downstream of exon 3 of the hematopoietically expressed homeobox (Hhex) gene. The construct was electroporated into 129S1/Sv embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene, and offspring were bred to C57BL/6NCrl mice for at least 6 generations. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
Allele Name | targeted mutation 2, Clifford W Bogue |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Hhexflox |
Gene Symbol and Name | Hhex, hematopoietically expressed homeobox |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv |
Chromosome | 19 |
Molecular Note | A FRT-flanked neo cassette followed by a loxP site was inserted in the first intron of the targeted gene. An additional loxP site was added downstream of exon 3. Mice were bred to Flpe-expressing mice to excise the neo cassette, leaving exons 2 and 3 flanked by loxP sites. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Hhexflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #025396 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Hhex<tm2Cwb> |
Frozen Mouse Embryo | B6N.129S1(Cg)-Hhex<tm2Cwb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1(Cg)-Hhex<tm2Cwb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1(Cg)-Hhex<tm2Cwb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129S1(Cg)-Hhex<tm2Cwb>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.