C57BL/6N-Ifngr1^tm1.1Rds/J

Stock number: 025394
Product name: Ifngr1^f (IFN-γR floxed)

Description

These floxed mutant mice possess loxP sites flanking exon 3 and 4 of the Ifngr1 gene. This strain may be useful for generating conditional mutations in applications related to innate immunity.

Mouse mutants involving this gene have been used in studies of Zika virus pathogenesis.

Detailed description

These mice possess loxP sites on either side of exons 3 and 4 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissues. Cell surface expression of IFNGR1 unaltered, as determined with flow cytometry analysis of spleen, peripheral blood leukocytes or thymus.

When bred to a strain with Cre recombinase expression in dendritic cells (see Stock No. 007567 for example), this mutant mouse strain may be useful in studies of susceptibility to bacterial infections.

Development

A targeting vector containing a floxed neomycin resistance cassette was utilized in the construction of this mutant. This selection cassette inserted upstream of exon 3, and another loxP site was inserted downstream of exon 4. This construct was electroporated into C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley derived B6/BLU embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector (pTurbo-cre) to remove the selection cassette. Correctly targeted ES cells in which Cre recombination resulted in exons 3 and 4 being flanked by loxP sites, and that no longer contained the selection cassette, were microinjected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were maintained on a C57BL/6NTac background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Allele

Symbol: Ifngr1^tm1.1Rds
Name: targeted mutation 1.1, Robert D Schreiber
MGI accession ID: MGI:5558045
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ifngr1
Marker name: interferon gamma receptor 1
Chromosome: 10
Strain of origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley
Molecular note: A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed the selection cassette and left exons 3 and 4 floxed.