This Lamc2 knock out strain may be useful in studies of epidermolysis bullosa and is a model for Herlitz Type Junctional Epidermolysis Bullosa.
Dr. Derry Roopenian, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Lamc2 | laminin, gamma 2 |
The Lamc2 gene encodes for the gamma-2 subunit of laminin 332 (laminin 5), which is an extracellular matrix glycoprotein found in cutaneous basement membranes. These mice carry a knock out mutation of Lamc2 which results in a frameshift deletion of exon 8. Mice that are homozygous for the targeted mutation die by 5 days of age. Heterozgyotes are viable and fertile. No protein gene product is detected by immunofluorescence screening of skin from homozygotes. A truncated 291bp transcript is detected by RT-PCR analysis of skin from homozygotes and heterozygotes. Onset of the progressive blistering phenotype occurs within 1 to 2 days of birth in almost all homozygous mice. Dermal-epidermal tissue separation is found in the skin and mucous membranes (oral mucosa, urinary bladder).
A loxP site flanked targeting vector designed containing a NEO-TK cassette was utilized in the construction of this mutant. This selection cassette and a FRT site was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted upstream of exon 8. A second FRT site was inserted downstream of exon 9. The construct was electroporated into unspecified embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette or exon 8 were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. The mice were then backcrossed to C57BL/6J for 13 generations. Upon arrival at The Jackson Laboratory Repository, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, Jouni Uitto |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Lamc2- |
Gene Symbol and Name | Lamc2, laminin, gamma 2 |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 1 |
Molecular Note | A floxed neomycin resistance gene was inserted in intron 8 and another loxP site was placed in intron 7. Also, frt sites were inserted on either side of exon 9. Transient cre expression in the ES cells removed exon 8 which caused a frame shift and a premature termination codon in exon 9. RT-PCR indicated mRNA of 510bp rather than 623bp. There was also a minor smaller transcript of 291bp. No protein product could be seen immunologically in skin biopsies of homozygous mice. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes die by 5 days of age.
When using the B6.Cg-Lamc2tm1Uit/DcrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #025335 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wild-type for Lamc2<tm1Uit> |
Frozen Mouse Embryo | B6.Cg-Lamc2<tm1Uit>/DcrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Lamc2<tm1Uit>/DcrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Lamc2<tm1Uit>/DcrJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Lamc2<tm1Uit>/DcrJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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