These Rosa-STOP-mMFN mice express a Cre recombinase-inducible mutant human MFN2 containing the T105M mutation and may be useful in studies related to Charcot-Marie-Tooth neuropathy.
David Pleasure, UC Davis
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence, No functional change) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
The Mfn2 gene encodes a mitochondrial membrane GTPase involved in mitochondrial fusion and regulation of vascular smooth muscle cell proliferation. These mice carry a knock in allele of a mutant human MFN2 gene in which a floxed STOP-NEO cassette, a human MFN2 cDNA carrying the T105M mutation (GTPase domain), and human growth hormone poly A sequences under the control of the CAG promoter was inserted into the Gt(ROSA)26Sor locus. Due to the floxed STOP cassette, expression of the mutant MFN2 is Cre recombinase dependent. Heterozygotes are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous. Further characterization of this strain is currently underway. We will supplement the strain description as published results become available.
A targeting vector containing a floxed STOP-NEO cassette, a human MFN2 cDNA carrying the T105M mutation, and human growth hormone poly A sequences under the control of the CAG promoter was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into C57BL/6 derived embryonic stem (ES) cells (genOway).
Correctly targeted ES cells were injected into albino C57BL/6 blastocysts (derived from C57BL/6J-Tyrc-2J/J). The resulting male chimeras were bred to wildtype C57BL/6J females. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | MFN2, mitofusin 2, human |
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Site of Expression |
Allele Name | targeted mutation 1, David Pleasure |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence, No functional change) |
Allele Synonym(s) | Rosa-STOP-MFN2T105M |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | MFN2, mitofusin 2, human |
Strain of Origin | C57BL/6 |
Chromosome | 6 |
Molecular Note | A targeting vector containing a floxed STOP-NEO cassette, a human MFN2 cDNA carrying the T105M mutation, and human growth hormone poly A sequences under the control of the CAG promoter is inserted into the Gt(ROSA)26Sor locus. |
When maintaining a live colony, these mice can be bred as heterozygotes. The Donating Investigator has not attempted to make the strain homozygous.
When using the Rosa-STOP-mMFN mouse strain in a publication, please cite the originating article(s) and include JAX stock #025322 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gt(ROSA)26Sor<tm1(CAG-MFN2*T105M)Dple> |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(CAG-MFN2*T105M)Dple>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(CAG-MFN2*T105M)Dple>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(CAG-MFN2*T105M)Dple>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(CAG-MFN2*T105M)Dple>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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