C57BL/6-Gt(ROSA)26Sor^{tm1(CAG-MFN2*T105M)Dple}/J

Stock number: 025322
Product name: Rosa-STOP-mMFN
Research areas: Neurobiology Research, Research Tools

Description

These Rosa-STOP-mMFN mice express a Cre recombinase-inducible mutant human MFN2 containing the T105M mutation and may be useful in studies related to Charcot-Marie-Tooth neuropathy.

Detailed description

The Mfn2 gene encodes a mitochondrial membrane GTPase involved in mitochondrial fusion and regulation of vascular smooth muscle cell proliferation. These mice carry a knock in allele of a mutant human MFN2 gene in which a floxed STOP-NEO cassette, a human MFN2 cDNA carrying the T105M mutation (GTPase domain), and human growth hormone poly A sequences under the control of the CAG promoter was inserted into the Gt(ROSA)26Sor locus. Due to the floxed STOP cassette, expression of the mutant MFN2 is Cre recombinase dependent. Heterozygotes are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous. Further characterization of this strain is currently underway. We will supplement the strain description as published results become available.

Development

A targeting vector containing a floxed STOP-NEO cassette, a human MFN2 cDNA carrying the T105M mutation, and human growth hormone poly A sequences under the control of the CAG promoter was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into C57BL/6 derived embryonic stem (ES) cells (genOway). Correctly targeted ES cells were injected into albino C57BL/6 blastocysts (derived from C57BL/6J-Tyr^c-2J/J). The resulting male chimeras were bred to wildtype C57BL/6J females. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Gt(ROSA)26Sor^{tm1(CAG-MFN2*T105M)Dple}
Name: targeted mutation 1, David Pleasure
MGI accession ID: MGI:5629288
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence; Humanized sequence; No functional change
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: C57BL/6
Expressed genes: MFN2,mitofusin 2,human
Molecular note: A targeting vector containing a floxed STOP-NEO cassette, a human MFN2 cDNA carrying the T105M mutation, and human growth hormone poly A sequences under the control of the CAG promoter is inserted into the Gt(ROSA)26Sor locus.