This albumin-deficient model may be useful for studying the biology and pathobiology of serum albumin as well as evaluating human albumin pharmacokinetics.
Michael Wiles, The Jackson Laboratory
This TALEN-mediated deletion in Alb results in the absence of any albumin protein in the blood. Heterozygous and homozygous mice do not exhibit any gross abnormal phenotype. This strain may be useful as a control with B6.Cg-Albem12Mvw Fcgrttm1Dcr Tg(FCGRT)32Dcr/MvwJ (Stock No. 025201) for studying the biology and pathobiology of serum albumin as well as evaluating human albumin pharmacokinetics.
A targeting scheme was designed using a TALEN-mediated Non-Homologous End Joining (NHEJ) repair pathway to knock out the albumin gene in C57BL/6J (Stock No. 000664). TALEN mRNAs were introduced into C57BL/6J zygotes, and the resulting founder mouse (#5074) was crossed to a wild-type C57BL/6J to characterize the mutation, a single base deletion resulting in early termination of the translated protein. N1 Heterozygous offspring were filial-crossed to establish the colony as homozygotes.
|Allele Name||endonuclease-mediated mutation 8, Michael Wiles|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Alb, albumin|
|Strain of Origin||C57BL/6J|
|Molecular Note||Exon 4 was targeted by introduction of TALEN mRNA into fertilized C57BL/6J oocytes and the resulting founder screened and found to have a 1 base pair deletion at Chromosome 5 base 90,464,113 (GRCm38.p2) which is predicted to result in a frame shift then a premature stop codon 55 amino acids downstream of the encoded frame shift. By ELISA, heterozgyotes have a decrease in serum albumin of approximately 30 percent and homozygotes have no detectable serum albumin.|
While maintaining a live colony, these mice are bred as homozygotes x heterozygotes or reciprocal. Homozygotes x homozygotes units tend to be nonproductive although the few productive units produce smaller litters.
When using the C57BL/6J-Albem8Mvw/MvwJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #025200 in your Materials and Methods section.
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