These mice coexpress tau-lacZ from pheromone receptor Vmn1r51 enabling the analysis of sensory neuron projection patterns.
Catherine Dulac, Harvard University, HHMI
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Vmn1r51 | vomeronasal 1 receptor 51 |
Vmn1r51 (vomeronasal 1 receptor 51; also called VN12) is a member of the vomeronasal organ V1R gene family, whose activation is believed to be involved with pheromone detection.
These mice co-express tau-lacZ with pheromone receptor Vmn1r51 (vomeronasal 1 receptor 51; also called VN12) enabling the analysis of sensory neuron projection patterns. This strain provides insight on the workings of olfactory coding in the vomeronasal system. Analysis of whole mounts of the nasal cavity dissected from adult homozygous mice reveals a subpopulation of vomeronasal sensory neurons whose dendrites, cell bodies, and axons display intense blue color after staining with X-gal.
A IRES-tau-lacZ-loxP-Herpes simplex virus thymidine kinase (HSV-TK)-neomycin-loxP cassette was placed immediately 3' of the stop codon via homologous recombination in 129-derived embryonic stem (ES) cells. A targeted ES cell clone was transiently infected with a vector driving expression of Cre recombinase in order to delete the HSV-TK and neomycin sequences, leaving a single loxP site. This strain was maintained on a mixed C57BL/6-129 genetic background by the donating laboratory.
An allele-specific genotyping assay has not been developed for this strain.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | tau-lacZ is expressed in vomeronasal sensory neurons. |
Allele Name | targeted mutation 2.1, Catherine Dulac |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | VN12-IRES-tau-lacZ |
Gene Symbol and Name | Vmn1r51, vomeronasal 1 receptor 51 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | tau-lacZ is expressed in vomeronasal sensory neurons. |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | An IRES-tau-lacZ-loxP-Herpes simplex virus thymidine kinase (HSV-TK)-neomycin-loxP cassette was placed immediately 3' of the stop codon. Cre-mediated recombination removed the HSV-TK and neomycin sequences, leaving a single loxP site. |
Homozygotes and heterozygotes are viable and fertile.
When using the B6;129-Vmn1r51tm2.1Dlc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025196 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Vmn1r51<tm2.1Dlc> |
Frozen Mouse Embryo | B6;129-Vmn1r51<tm2.1Dlc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Vmn1r51<tm2.1Dlc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Vmn1r51<tm2.1Dlc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Vmn1r51<tm2.1Dlc>/J Frozen Embryo | $3373.50 |
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