These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Vangl2 gene. This strain may be useful for generating conditional mutations in applications related to planar cell polarity.
Micheal Deans, Johns Hopkins Univ. School of Medicine
The Vangl2 gene encodes for a membrane protein that plays a role in planar cell polarity, and is critical in the organization of the stereociliary bundles of the cochlea.
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in the developing otic placode, this mutant mouse strain may be useful in studies of planar cell polarity in the cochlea.
A targeting vector containing an FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to ACTB–FlpE transgenic mice (on an unspecified genetic background) expressing FLP recombinase under the control of the human ACTB promoter. Mice that retained the loxP site flanked exons 2 and 3 were then bred to B6129PF1/J (Stock No. 100492) mice to remove the ACTB–FlpE transgene. Heterozygotes were crossed to generate homozygotes.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 2.1, Michael R Deans|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Vangl2, VANGL planar cell polarity 2|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the resistance cassette and left exons 2 and 3 floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Vangl2tm2.1Mdea/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025174 in your Materials and Methods section.