These transgenic mice express a tamoxifen-inducible cre recombinase, Cre-ERT2, under the control of the Adipoq gene promoter. They may be useful in generating adipocyte-specific targeted mutants in studies related to adipose tissue function and storage, obesity, and other metabolic diseases.
Stefan Offermanns, Max-Planck-Institute for Heart and Lung
Genetic Background | Generation |
---|---|
N13+pN2F11
|
Allele Type |
---|
Transgenic (Recombinase-expressing, Inducible) |
Starting at:
$270.00 Domestic price for female 4-week |
348.51 Domestic price for breeder pair |
Mice hemizygous for this Adipoq-CreERT2 BAC transgene are viable and fertile, with expression of a tamoxifen inducible Cre recombinase (cre/ERT2) directed to adipose tissue by the promoter/regulatory regions of the mouse adiponectin (Adipoq) locus on the BAC transgene. When crossed with a Rosa26LacZ reporter tamoxifen induced Cre recombination is detected in 97%–99% of adipocytes in white adipose tissue and approximately 15% of adipocytes in brown adipose tissue. No tamoxifen induced Cre recombination is detected in liver, pancreas, gut, kidney, lung, aorta, skeletal muscle, heart, spleen, thymus, cerebrum, or cerebellum. Cre recombinase activity is not detected in the absence of tamoxifen.
The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
A targeting construct containing sequence encoding Cre-ERT2 fusion protein and a FRT site flanked ampicillin resistance cassette was utilized to generate this mutant strain. The mouse bacterial artificial chromosome (BAC) BAC RP23-112M7, containing the entire Adipoq gene (as well as Kng1, Eif4a2, and Rfc4), was modified by insertion of this targeting vector into the start codon of Adipoq. The FRT flanked selection cassette by excised by Flp recombinase. A transgenic construct was generated from the modified BAC and microinjected into C57BL/6 oocytes.
Founder line 1 was subsequently established. The donating investigator reported that the mice were then backcrossed to C57BL/6 for 13 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
---|---|
Site of Expression | lacZ is expressed in epididymal, mesenterial, and retroperitoneal white adipose tissue after tamoxifen treatment. |
Allele Name | transgene insertion 1, Stefan Offermanns |
---|---|
Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Adipoq creER(T)2; AdipoqCreERT2 |
Gene Symbol and Name | Tg(Adipoq-cre/ERT2)1Soff, transgene insertion 1, Stefan Offermanns |
Gene Synonym(s) | |
Promoter | Adipoq, adiponectin, C1Q and collagen domain containing, mouse, laboratory |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | lacZ is expressed in epididymal, mesenterial, and retroperitoneal white adipose tissue after tamoxifen treatment. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A targeting vector containing cDNA encoding cre recombinase fused to the mutated form of the ligand binding domain of the human estrogen receptor followed by an Frt-flanked ampicillin resistance marker was generated. Homologous recombination resulted in insertion of the vector into the start codon of the Adipoq locus present in a BAC clone (RP23-112M7; this BAC also contained the genes Kng1, Eif4a2, and Rfc4.) Flp-mediated excision removed the ampicillin marker. Five founders were obtained and crossed with lacZ reporter mice. Progeny of one founder displayed homogeneous lacZ expression in epididymal, mesenterial, and retroperitoneal white adipose tissue after tamoxifen treatment. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.
When using the C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025124 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizyous or Non Carrier for Tg(Adipoq-cre/ERT2)1Soff |
Frozen Mouse Embryo | C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.