Overview

Also Known As: Foxp3^EGFP

These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3, with expression of EGFP restricted to the T cell lineage, primarily to the CD4+ T reg cell population. On the congenic NOD background these mutant mice exhibit a diabetes incidence similar to NOD inbred mice. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.

Donating Investigator

Dr. David Serreze, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Foxp3^tm2Tch

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Foxp3	forkhead box P3

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Research Applications

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Homozygous mice are viable and fertile with normal T and B cell development. These "Foxp3^EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3⁺ T cell population (more than 97% of Foxp3⁺ T cells were EGFP⁺), and Foxp3 mRNA expression strictly segregates with EGFP⁺ T cells. Due to X-inactivation in females, when the reporter construct in a heterozygous not a homozygous state, the number of EGFP⁺CD4⁺ T cells found in the peripheral blood of heterozygous females was approximately half that of hemizygote males. CD4⁺EGFP⁺ cells also exhibit normal regulatory T cell suppression of effector cell proliferation (following stimulation with anti-CD3 and anti-CD28 monclonal antibodies ). Some EGFP expression is noted in a small population of CD8⁺ thymocytes. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response. On the congenic NOD background these mutant mice exhibit a diabetes incidence similar to NOD inbred mice.

This strain targets the GFP cassette downstream of the endogenous Foxp3 STOP codon with no defects in Foxp3 or regulatory T cell function observed.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This allele was originally published on a BALB/c genetic background (see Stock No. 006769). It should be noted that the phenotype could vary from that originally described. The strain description will be modified as published results become available.

Development

A targeting vector was designed to place an IRES-EGFP-SV40 polyA sequence immediately downstream of the endogenous Foxp3 translational stop codon but upstream of the endogenous polyA signal. This also placed a loxP-flanked PGKneo cassette into intron 9. This vector was electroporated into 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with C57BL/6 females. The PGKneo cassette was removed by mating founder males with Cre-deleter female mice on a C57BL/6 congenic background. The resulting mice (now harboring this Foxp3EGFP allele) were maintained on a B6 background prior to 18 backcross generations to NOD/ShiLtDvs. Upon transfer to The Jackson Laboratory Repository, the mice were crossed to NOD/ShiLtJ (Stock No. 001976) at least once to establish the colony.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in the Foxp3⁺ T cell population. Some EGFP expression is noted in a small population of CD8⁺ thymocytes.

Control Suggestions

Approximate Controls

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

Haribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72PubMed: 17312141 MGI: J:120713

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Genetics

Foxp3^tm2Tch

Allele Symbol: Foxp3^tm2Tch

Allele Name	targeted mutation 2, Talal A Chatila
Allele Type	Targeted (Reporter)
Allele Synonym(s)	Foxp3^tm2Tch; targeted mutation 2, Talal A Chatila
Gene Symbol and Name	Foxp3, forkhead box P3
Gene Synonym(s)	AIID; sf; DIETER; IPEX; PIDX; scurfin; XPID; JM2; scurfy; RGD1562112
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in the Foxp3⁺ T cell population. Some EGFP expression is noted in a small population of CD8⁺ thymocytes.
Strain of Origin	129X1/SvJ
Chromosome	X
Molecular Note	An IRES-EGFP-SV40 poly A sequence was inserted immediately downstream of the endogenous Foxp3 translational stop codon, but upstream of the endogenous polyA signal, generating a bicistronic locus encoding both Foxp3 and EGFP. Expression of EGFP is restricted to the T cell lineage, primarily to the CD4+ T cell population.
Mutations Made By	Talal Chatila, Boston Children's Hospital

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Haribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72PubMed: 17312141 MGI: J:120713

Additional - Foxp3^tm2Tch related

Technical Support

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Genotyping Protocols
- High Resolution Melting: Foxp3^tm2Tchalternate1
- Genotyping resources and troubleshooting
Breeding Considerations

This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females are bred with hemizygous males.
- Additional Breeding and Husbandry Support
Citation
When using the Foxp3^EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #025097 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	X linked - females are Heterozygous and males are wildtype for Foxp3<tm2Tch>, 1 pair minimum

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Foxp3EGFP

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Foxp3tm2Tch

Allele Symbol: Foxp3tm2Tch

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Foxp3tm2Tch related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: Foxp3^EGFP

Foxp3^tm2Tch

Allele Symbol: Foxp3^tm2Tch

Additional - Foxp3^tm2Tch related